Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 549412, 13 pages
http://dx.doi.org/10.1155/2015/549412
Review Article

Hypoxia-Inducible Factor-1 in Physiological and Pathophysiological Angiogenesis: Applications and Therapies

Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznań, Poland

Received 19 December 2014; Revised 10 April 2015; Accepted 17 April 2015

Academic Editor: Alessio D’Alessio

Copyright © 2015 Agnieszka Zimna and Maciej Kurpisz. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The cardiovascular system ensures the delivery of oxygen and nutrients to all cells, tissues, and organs. Under extended exposure to reduced oxygen levels, cells are able to survive through the transcriptional activation of a series of genes that participate in angiogenesis, glucose metabolism, and cell proliferation. The oxygen-sensitive transcriptional activator HIF-1 (hypoxia-inducible factor-1) is a key transcriptional mediator of the response to hypoxic conditions. The HIF-1 pathway was found to be a master regulator of angiogenesis. Whether the process is physiological or pathological, HIF-1 seems to participate in vasculature formation by synergistic correlations with other proangiogenic factors such as VEGF (vascular endothelial growth factor), PlGF (placental growth factor), or angiopoietins. Considering the important contributions of HIF-1 in angiogenesis and vasculogenesis, it should be considered a promising target for treating ischaemic diseases or cancer. In this review, we discuss the roles of HIF-1 in both physiological/pathophysiological angiogenesis and potential strategies for clinical therapy.

1. Angiogenesis

The circulatory system is the first biological system that is established during mammalian development [1]. Vessel formation occurs via only two basic mechanisms: vasculogenesis and angiogenesis [2]. During embryonic development, the primary vascular plexus is formed by vasculogenesis. This phenomenon involves de novo blood vessel formation from precursor cells called angioblasts (precursors of endothelial cells), whereas angiogenesis is the process by which blood vessels are formed from preexisting vessels. This process involves the remodelling of blood vessels into the large and small vessels that are typical for networks containing arteries, capillaries, and veins [3, 4]. Angiogenesis occurs in adult organisms and in embryos during development [3].

Angiogenesis consists of several basic steps. Briefly, biological signals such as hypoxia, ischaemia, and/or blood vessel damage upregulate the expression of proangiogenic growth factors that activate their receptors [5, 6]. Vascular permeability increases in response to VEGF, thereby allowing the extravasation of plasma proteins that form a primitive scaffold for migrating endothelial cells [7]. Angiopoietin-1 and angiopoietin-2 (Ang-1 and Ang-2) exert antagonistic functions during vessel development. Ang-1, which is a known natural inhibitor of vascular permeability, protects against plasma leakage, whereas Ang-2 is involved in vessel destabilisation via the detachment of smooth muscle cells and the promotion of permeabilisation [8, 9]. Subsequently, matrix metalloproteinases (MMPs) enhance angiogenesis through the degradation of matrix components [10]. Proliferating endothelial cells migrate to distant sites and then assemble as a solid cord that subsequently forms a lumen [11]. Integrins αβ promote endothelial cell adhesion and migration, whereas VE-cadherin increases cell survival and promotes endothelial cell adhesion [1214]. Once the vessels are formed, pericytes and smooth muscle cells surround the newly created capillaries to stabilise the walls and to prevent leakage. Other factors such as Ang-1, PDGF-BB (platelet-derived growth factor BB), and PDGFR (platelet-derived growth factor receptor) participate in the maturation of blood vessels [8, 15]. Further expansion of the lumen diameter is called arteriogenesis [11].

Angiogenesis occurs in physiological states such as embryonic development, wound healing, or vessel penetration into avascular regions and in pathological states such as solid tumours formation, eye diseases, or chronic inflammatory disorders such as rheumatoid arthritis, psoriasis, and periodontitis [16, 17]. Pathophysiological angiogenesis exhibits differences in molecular pathways in comparison to physiological angiogenesis. Mutations in oncogenes and tumour suppressor genes and disruptions in growth factor activity play crucial roles during tumour angiogenesis [17]. The activation of the most prominent proangiogenic factor VEGF might be due to physiological stimuli such as hypoxia or inflammation or due to oncogene activation and tumour suppression function loss [18, 19]. Additionally, physiological angiogenesis such as that which occurs during embryonic development or wound healing seems to be dependent on VEGF signalling, whereas tumour angiogenesis adopts the ability to shift its dependence from VEGF to other proangiogenic pathways, for example, through the recruitment of myeloid cells and the upregulation of alternative vascular growth factors (PlGF and FGF, fibroblast growth factor) [17]. Moreover, tumour vessels are distinct from normal vasculature because they are disorganised and tortuous. Many morphological and functional differences exist between normal and tumour vasculature. For instance, tumour vessels are leakier than normal vessels, and endothelial cells growing within tumours carry genetic abnormalities [20, 21]. Elucidating the molecular mechanism of pathological angiogenesis might lead to the identification of potential therapeutic targets.

Angiogenesis is a multistep process that requires the involvement of many biological signals and stimuli regardless of whether it is a physiological or pathological action. Proangiogenic factors are activated in response to some physical signals. Blood vessel damage, infarction, and blood flow reduction lead to decreases in O2 supply [11]. This state is called hypoxia, which is a potent angiogenic trigger that stimulates proangiogenic factor activity.

2. Hypoxia

2.1. O2 Homeostasis

Constant oxygen supply is essential for proper tissue function, development, and homeostasis. Thus, the vasculature network plays a crucial role in delivering oxygen particles (O2), nutrients, and other molecules within the entire human body [22]. Significantly, the first physiological system that becomes functional during mammalian embryonic development is the circulatory system. Governing O2 homeostasis in tissues by supplying an oxygen concentration adequate for the demand generated by the metabolic outputs of the tissue is essential. Oxygenic balance can be upset by rapid cellular division during embryonic development, by tumour growth, or by vasculature dysfunction due to vessel occlusion or rupture [23]. Notably, normal physiological O2 concentrations vary greatly from normal oxygen tension in the air depending on the type of tissue. For instance, arterial blood has a normal pO2 of 14%; myocardium, 10%; and skeletal muscle, 5%. In contrast, the natural pO2 levels of bone marrow, thymus, and cartilage are at or below 1% [24, 25]. The state when the O2 level decreases relative to physiological levels (characteristic for particular tissues) is called hypoxia [1].

2.2. Hypoxia-Inducible Factor-1

Adaptation to low oxygen tension (hypoxia) in cells and tissues requires the activation of several genes that participate in angiogenesis, cell proliferation/survival, glucose and iron metabolism. In eukaryotic cells, hypoxia-inducible factor-1 (HIF-1) is a primary transcriptional mediator of the hypoxic response and master regulator of O2 homeostasis [26]. Hypoxia-inducible factor-1 was first discovered as a transcription factor that regulates erythropoietin (EPO) expression in response to low oxygen levels in the blood [27, 28]. HIF-1 consists of two different subunits, α and β (also known as aryl hydrocarbon nuclear receptor translocator (ARNT)); both subunits are members of the basic helix-loop-helix Per-Arnt-Sim (bHLH-PAS) transcription factor family [29, 30]. PAS and bHLH motifs are required for heterodimerisation between the HIF-1α and HIF-1β subunits [31]. Additionally, the bHLH domain of the HIF-1α/ARNT dimer is essential for DNA binding on hypoxia response elements (HREs) with the consensus sequence (G/ACGTG) in the promoters or enhancers of target genes [32]. Transcriptional activation and interactions with coactivators (such as CBP/p300) of HIF-1α are mediated by two domains, that is, C-TAD and N-TAD, which are on the C-terminus of the protein [3335]. HIF-1β is a constitutively expressed subunit, whereas HIF-1α is translated continuously and degraded subsequently through ubiquitination under normoxic conditions [36, 37]. Thus far, three isoforms of HIF-α have been discovered (Figure 1(a)), that is, the previously discussed subunit HIF-1α and two other subunits, HIF-2α (also called endothelial PAS protein (EPAS)) and HIF-3α (IPAS). The second isoform is expressed constitutively in the endothelium, lung, and cartilage and shares 48% amino acid sequence identity with HIF-1α. The third protein, HIF-3α, also called inhibitory PAS (IPAS), is a negative regulator of HIF-1 that dimerises with the HIF-1α subunit and prevents its DNA-binding activity. The entire set of HIF-α subunits dimerises with ARNT and binds to HREs [3840].

Figure 1: Schematic representation of HIF-α gene structures and DNA binding. (a) HIF-1α and HIF-2α contain the following domains: a nuclear localisation domain (NLS), DNA binding and dimerisation domains (bHLH/PAS), oxygen-dependent degradation domain (ODDD), and cofactor interaction and transcriptional activity domains (N-TAD/C-TAD). HIF-3α lacks a C-TAD domain. NLS: nuclear localisation signal; bHLH: basic helix-loop-helix domain; PAS: Per-ARNT-Sim motif; ODDD: oxygen-dependent degradation domain; N-TAD: N-terminal transactivation domain; C-TAD: C-terminal transactivation domain. (b) Dimerisation of HIF-1α with HIF-1β under hypoxic conditions results in the formation of the HIF-1 transcription factor, which binds to hypoxia response elements (HREs) and activates the transcription of O2-dependent genes (according to [134]).

Under extended exposure to hypoxic conditions, HIF-1α is expressed as long as a balance between O2 supply and usage in tissues cannot be reached [36, 37]. When oxygen tension is sufficient, de novo synthesised cytoplasmic HIF-1α is hydroxylated by a family of prolyl hydroxylase enzymes (PHDs) on proline 402 and 564 residues located within ODDD (O2-dependent degradation domain) [41, 42]. The hydroxylation of the abovementioned prolines is the key mechanism of negative regulation of HIF-1α activity and results in the binding of the von Hippel-Lindau (VHL) E3 ligase complex, which ubiquitinates HIF-1α, targeting it to proteasomal degradation [4345]. The second major mechanism that modulates HIF-1α activity is the hydroxylation of asparagine residue 803 (Asn803) in the C-TAD domain. In this case, asparagine is hydroxylated under normoxic conditions by factor inhibiting HIF-1 (FIH-1), which prevents the interaction of HIF-1α within CBP/p300 (CREB-binding protein/E1A binding protein p300) [4649]. Hydroxylases (PHDs and FIH-1) are strictly Fe(II)- and 2-oxoglutarate-dependent dioxygenases that are activated only in the presence of molecular oxygen. Under hypoxic conditions, substrates and coactivators of hydroxylation such as O2, Fe(II), and 2-oxoglutarate become limited, which leads to the attenuation of HIF-1α hydroxylation [50, 51]. HIF-1α accumulates in the cytosol and is subsequently translocated into the nucleus where it dimerises with the HIF-1β subunit. The HIF-1α/β dimer binds to HREs that are located within O2-regulated genes (Figure 2) [34, 35]. HIF-1 target gene members include a stress response gene family that mediates the adaptation of cells/tissues to chronic or acute hypoxia; this family includes glucose transporters, glycolytic enzymes, and angiogenic and haematopoietic growth factors [52].

Figure 2: HIF-1α under normoxic and hypoxic conditions. In the presence of molecular oxygen, 2-oxoglutarate, and Fe2+, HIF-1α is hydroxylated on proline 402 and 564 residues located within ODDD (O2-dependent degradation domain) by prolyl hydroxylase enzymes (PHDs). Hydroxylation results in the binding of the von Hippel-Lindau (VHL) E3 ligase complex, which ubiquitinates HIF-1α, targeting it to proteasomal degradation. The hydroxylation of the asparagine residue prevents CBP/p300 binding to HIF-1α. Under hypoxic conditions, substrates and coactivators of hydroxylation such as O2, Fe(II), and 2-oxoglutarate become limited, which leads to the attenuation of HIF-1α hydroxylation. HIF-1α accumulates in the cytosol and is subsequently translocated into the nucleus where it dimerises with the HIF-1β subunit. The HIF-1α/β dimer binds to HREs and regulates target gene expression.
2.3. HIF-1 Activity and Target Genes

Recently, HIF-1 has been shown to regulate more than 2% of genes in vascular endothelial cells either directly or indirectly [53]. The modulation of cell responses is followed by the transcriptional activation of target genes by the HIF-1α/β dimer (Figure 1(b)) [54, 55]. For example, hypoxia upregulates the expression of erythropoietin (EPO), which is required for red blood cell production. The generation of new erythrocytes increases the delivery of oxygen to tissues to reach O2 homeostasis [56]. Additionally, low oxygen levels influence glucose metabolism in cells. Under hypoxic conditions, cells generate only 2 ATP molecules by oxygen-independent glycolysis instead of 38 ATP molecules by the oxygen-dependent tricarboxylic acid cycle (TCA) under normoxic conditions [57, 58]. To maintain the energetic balance under hypoxic conditions, cells increase their ability to produce ATP by increasing glucose uptake via the enhancement of glycolytic enzyme and transporter expression [5961]. Furthermore, cell proliferation and survival may be enhanced under hypoxic conditions. Factors such as IGF-2 (insulin growth factor-2) and TGFα (transforming growth factor) are upregulated through HIF-1 activity [62, 63]. Additionally, myoblasts cultured under hypoxic conditions show increased proliferation compared to cells maintained under normoxic conditions. Myogenic gene expression analysis revealed that the genes MyoD and Myf5 (both involved in myogenic cell proliferation and differentiation) were upregulated in hypoxic cells, whereas no significant influence of myogenic gene expression was observed in normoxic cells [64].

3. Hypoxia-Induced Angiogenesis

3.1. Physiological Vasculogenesis and Angiogenesis under Hypoxic Conditions

Vasculogenesis is a characteristic embryonic process that involves de novo blood vessel formation and that leads to establishing a primary vascular plexus. O2 tension plays a crucial role in organogenesis and vasculogenesis during embryonic development [1]. During the first stage of embryogenesis, before the circulatory system develops, the oxygen tension is relatively low and does not exceed 3% [65, 66]. The developing embryo requires an increase in the oxygen level, which leads to the formation of primary vessels from angioblasts. Because the uterine environment is hypoxic it is thereby obvious that hypoxia may be the primary stimulus of vessel formation [3, 4]. Hypoxia also stimulates EC (endothelial cell) behaviour. Under in vitro conditions, HIF-1α promotes the arterial differentiation of endothelial progenitor cells (EPCs) over venous differentiation by regulating the expression of genes that inhibit the venous specification factor Coup-TFII (Hey2 and delta-like ligand 4 (DII4)) [67]. In contrast, HIF-1β enhances the differentiation of hemangioblasts from mesodermal progenitors [68]. Mouse model studies have demonstrated the participation of both HIF-1 dimer subunits in vasculogenesis [69, 70]. For instance, HIF-1α- or HIF-1β (ARNT) deficient mouse embryos showed aberrant placental architecture with fewer foetal blood vessels [7173]. Additionally, a lack of the HIF-1β gene results in defective vascular development in the yolk sac, branchial arches, cranium, and somites [74, 75]. These defects are lethal for embryos at day 10.5. HIF-1 subunit deficiencies markedly decrease VEGF mRNA and protein expression, leading to defects in blood vessel formation and neural fold termination [70, 74, 75].

Current studies have indicated that hypoxia and HIF-1 expression in adult organisms may contribute to angiogenesis in the following ways: by transcriptionally activating several angiogenic genes and their receptors (VEGF, PlGF, PDGFB, ANGPT1, and ANGPT2) [76, 77]; by regulating proangiogenic chemokines and receptors (SDF-1α, stromal cell derived factor 1α, and S1P, sphingosine-1-phosphate, and receptors CXCR4, C-X-C chemokine receptor type 4, and S1PRs, sphingosine-1-phosphate receptors), thus facilitating the recruitment of endothelial progenitor cells to the site of hypoxia [78]; and by enhancing EC proliferation and division (regulating genes involved in the cell cycle and DNA replication) [53]. Summarising these findings, we conclude that HIF-1 can orchestrate the process of angiogenesis.

HIF-1 participates in every step of angiogenesis. Cross activity between HIF-1 and proangiogenic factors is a basic relationship during capillary formation under hypoxia. Every step of the vessel formation cascade is supported by HIF-1. Notably, vascular endothelial growth factor isoforms (VEGF-A, VEGF-B, VEGF-C, and VEGF-D) are the primary factors that participate in angiogenesis [79], and angiogenesis initiated by hypoxia and by HIF-1 is often VEGF-dependent primarily because HIF-1 is a master stimulator of vascular endothelial growth factor. The sprouting of new vessels is a complex process that involves a wide range of proangiogenic factors and their receptors. Under hypoxic conditions, HIF-1 accumulation upregulates the principal proangiogenic factor VEGF directly [80, 81]. VEGF activity induces the expression of Flt-1 (fms-related tyrosine kinase) and KDR (kinase insert domain receptor) receptors [82, 83]. As long as the oxygen balance is disrupted, VEGF will bind its receptors and stimulate capillary outgrowth. Additionally, VEGF is known as a factor that enhances the expression of other proangiogenic factors such as PlGF and FGF. We can assume that when the blood flow is sufficient to supply oxygen to cells and tissues, HIF-1α will be degraded, thereby inhibiting VEGF gene and protein expression and stopping the entire cascade of proangiogenic factors. In conclusion, HIF-1 may regulate the expression of proangiogenic factors either directly (by binding to HREs) or indirectly (cascade effect) [84]. Aside from VEGFs, other factors participate in angiogenesis, including placental growth factor (PlGF), platelet-derived growth factor (PDGF), angiopoietins 1 and 2 (ANGPT1 and ANGPT2), and metalloproteinases (MMPs). Receptors such as Flt-1, KDR, Tie 1, and Tie 2 that transduce these signals and thereby maintain the cascade of new vessel formation are also important. Analyses of proangiogenic genes revealed the presence of HREs within the promoters of some genes [85, 86].

As was mentioned above, angiogenesis is a multistep process [87]. During the first step, hypoxia and HIF-1 stimulate VEGF and their receptors directly, and the cascade of new vessel creation begins. Second, the extracellular matrix must be degraded by metalloproteinases to allow the migrating endothelial cells to form tubes. Metalloproteinase 2 (MMP-2) expression has been shown to be enhanced by HIF-1α [88]. Next, integrins αβ (induced by HIF) [89] stimulate endothelial cell proliferation and adhesion, and HIF-1 controls EC behaviour. The Manalo group examined the influence of hypoxia and HIF-1 on the endothelial cell transcriptome. Microarray analysis demonstrated the upregulation of several genes that are responsible for the cell cycle and DNA replication. Additionally, it was proven that ECs cultured under low density and hypoxic conditions exhibit increased proliferation. In conclusion, HIF-1 can stimulate cell proliferation and division under hypoxia-induced angiogenesis by transcriptionally regulating the genes involved in the basic biological functions of cells [53]. Finally, the last step of angiogenesis is vessel maturation, which involves the recruitment of vascular supporting cells (pericytes and smooth muscle cells) and the formation of the basement membrane. In this case, HIF-2α is a primary enhancer of genes that determine blood vessel stabilisation. For example, fibronectin is a component of the basement membrane, and HIF-2α is involved in the upregulation of this gene [90]. Considering these findings, we can assume that HIF-1 regulates almost every step of capillary formation.

Increasing knowledge regarding the influence of HIF-1 on angiogenesis has been applied to in vitro studies that attempt to develop treatments for ischaemic diseases. For example, the adenoviral transfer of HIF-1α and HIF-2α into rabbit ischaemic limbs resulted in an increase in blood flow followed by induction of vessel sprouting. Additionally, these capillaries were highly enlarged compared to those of rabbits transduced with VEGF-A. As another consequence of VEGF gene transfer, the newly formed vessels were leaky, and the area of transfer was surrounded by oedema compared to animals treated with AdHIF-1α and AdHIF-2α [91]. In turn, in vivo studies demonstrated that the deletion of HIF-2α in murine ECs caused VEGF-induced acute vessel permeability. Furthermore, immortalised HIF-2α-deficient ECs exhibited decreased adhesion to extracellular matrix proteins and diminished expression of fibronectin, integrins, endothelin B receptor, angiopoietin-2, and delta-like ligand 4 (Dll4) [90]. Artificial hypoxia induced by CoCl2 (0.5 mM) stimulates angiogenic responses in SCAPs (stem cells from apical papilla) cocultured with HUVECs (human umbilical vein endothelial cells). Hypoxic conditions induced upregulated HIF-1α and VEGF expression in HUVECs, whereas ephrin-B2 gene was enhanced in SCAPs. Notably, this gene plays a central role in heart morphogenesis and angiogenesis by regulating cell adhesion and cell migration. Additionally, synergistic effects between HIF-1, VEGF, and ephrin-B2 led to an increase in the endothelial tubule number, vessel length, and branching points [92].

In conclusion, hypoxia-induced angiogenesis is a complex process that has been tested in both in vitro and in vivo studies. Moreover, the application of HIF-1 therapy in phase I clinical studies of critical limb ischaemia resulted in complete recovery of the ischaemic region [93]. Increased knowledge of the role of HIF-1 in angiogenesis may provide promising treatment methods for ischaemic diseases as partly described in Section 4.1 of this paper.

3.2. The Effect of Hypoxia on Pathophysiological Angiogenesis and the Role of HIF-1 in Tumour Angiogenesis

Low oxygen tension has been linked with many pathophysiological disorders and human diseases. Hypoxia is a component of tumour development and metastasis, while angiogenesis is principal for tumour growth and progression [94, 95]. Current studies have suggested that in terms of malignant transformation the activation of HIF-dependent angiogenesis in cancer occurs in two basic ways: by hypoxic conditions prevailing in the tumour cell mass or by genetic alterations caused by tumour transformation, genetic disorders, or molecular interactions that stimulate HIF activity, irrespective of oxygen tension. Undeniably, HIF plays a critical role in stimulating angiogenesis. However, notably, proangiogenic activity is directly linked with HIF-dependent VEGF activation, which results in an “angiogenic switch” in growing tumour masses [96].

Hypoxia is best characterised as an HIF activator. When intensively proliferating cells form a solid tumour, the balance between oxygen supply and demand is impaired; therefore, hypoxic environments (intratumoural hypoxia) prevail in growing cell masses. This prevalence is the reason why newly activated HIF-1α is not ubiquitinated and targeted to proteasomal degradation. Accumulating HIF-1 upregulates the expression of a number of proangiogenic genes including VEGF and their receptors Flt-1, Flk-1, Ang-1, Ang-2, and Tie-2 receptor, and all of these genes are essential for sprouting new vessels. Among these factors, VEGF is considered a primary effector of tumour angiogenesis. Moreover, the expression of VEGF can enhance the expression of other proangiogenic factors and their receptors; thus, vessel outgrowth is stimulated by multiple factors [95, 97]. This phenomenon, which is called “angiogenic switching,” allows tumour cells to induce angiogenesis, thereby stimulating tumour progression by supplying oxygen and nutrients through the newly created capillaries [96].

The above-presented data suggest that hypoxia directly enhances angiogenesis by promoting VEGF expression. In contrast, HIF-1-dependent angiogenesis can be activated by factors other than hypoxia. HIF-1α accumulation does not always occur under low O2 tension. Thus, HIF-1 can also be regulated by oxygen-independent mechanisms. The malignant transformation of cells may cause a whole range of genetic alterations that block the ubiquitination and proteasomal degradation of HIF-1α [84, 98]. Carcinogenesis is linked with aberrations in tumour suppressor genes also known as antioncogene genes. The protein products of these genes may control cell division, the cell cycle, or apoptosis. When cells exhibit DNA damage, these proteins are responsible for repressing the cell cycle or cell division or for promoting apoptosis. The most important tumour suppressors are pRb, p53, p21, and PTEN. Some evidence has indicated that altered antioncogenes can enhance angiogenesis via HIF-dependent VEGF stimulation. For instance, deleting the p53 tumour suppressor gene in a human cancer cell line promotes the neovascularisation and growth of tumours in mice. This vascularisation was followed by increased HIF-1α levels and augmented HIF-1-dependent transcriptional activation of vascular endothelial growth factor (VEGF) [99]. Additionally, mutation in the tumour suppressor gene PTEN led to hypoxia-independent HIF-1α accumulation and to activated HIF-1-mediated proangiogenic gene expression [100]. In breast cancer, HER2 (receptor tyrosine kinase) signalling induces HIF-1α protein synthesis rather than inhibiting its degradation, thus manifesting a novel mechanism of HIF-1-dependent VEGF expression regulation [101]. These findings suggest that not only the hypoxic environment can lead to angiogenesis in the case of malignant transformation. Additionally, the genetic alteration of single gene may be a cause of hypoxia-independent stimulation of HIF. Some evidence has indicated that genetic diseases are involved in the activation of HIF and thereby the stimulation of angiogenesis. von Hippel-Lindau is a genetic disease that predisposes individuals to benign and malignant tumours [102]. This condition is defined by a mutation in the VHL gene; thus, pVHL is not translated. This protein is a ligase that ubiquitinates HIF-1α and causes its degradation by proteasomes; thus, maintaining the balance of HIF-1 under normoxia is crucial. A loss of pVHL allows HIF-1α to dimerise with HIF-1β and to activate the transcription of a number of proangiogenic genes, including VEGF. Patients suffering from this disease have frequent malignancies in the central nervous system and/or retinal hemangioblastomas or clear cell renal carcinomas [103106]. However despite homology between HIF-1α and HIF-2α there are evidences that in VHL-defective renal cell carcinoma HIF isoforms exhibit different or opposite effect on gene expression and proliferation of tumour cells [107]. Genetic predisposition is not the only factor that can stimulate oxygen-independent HIF angiogenesis involving VEGF gene activation. Cases where some other molecules can stimulate HIF-dependent angiogenesis have been demonstrated. As an example, we can recall a phenomenon based on the feedback loop of HIF-1 and a product of the anaerobic metabolic pathway. The abovementioned mechanism that activates HIF-1-dependent angiogenesis in cancer cells is the phenomenon called the Warburg effect. Because anaerobic conditions prevail in tumour cell masses, cancer cells produce energy primarily by glycolysis. As a side effect, cells also produce high levels of lactates and pyruvates. These molecules have been reported to increase HIF-1α accumulation and to regulate hypoxia-inducible gene expression, thus promoting VEGF transcription and translation and resulting in tumour angiogenesis [108].

Strong interactions between HIF-1α and VEGF lead to the rapid activation of the vessel formation cascade; however, these vessels exhibit several dysfunctions. The process of intratumoural vessel formation is a consequence of imbalanced activity of angiogenic activators and inhibitors. These vessels are not fully functional and exhibit structural abnormalities. A loss of stabilisation with pericytes and smooth muscle cells and a lack of an arterial and venous phenotype lead to leakage, poor blood flow, and perfusion [109, 110]. However, these vessels are able to deliver nutrients and oxygen to growing tumour cell masses. Thus, we can also conclude that tumours with vasculature are more sensitive to various therapies. Due to vascularisation, chemotherapeutics and inhibitors may be better distributed across tumour cells, thus leading to the inhibition of malignant cell growth.

4. Hypoxia as a Potential Therapeutic Tool

Hypoxia-inducible factor-1 has been considered a potential therapeutic target in many diseases. Predominantly, HIF-1 therapies focus on diseases common to developing countries such as ischaemic disorders (including cardiovascular disease and limb ischaemia) and cancer. Nonetheless, HIF-1 is also a potential therapeutic agent for treating endometriosis and blindness. Depending on the type of disease and the expected therapeutic effect, HIF-1 therapies have different approaches. The function of HIF-1 in therapies can be classified into two different strategies: HIF-1 upregulation (ischaemia) and HIF-1 inhibition (cancer and endometriosis) (Figure 3).

Figure 3: Different strategies in HIF-1α therapies. Depending on the type of disease and the expected therapeutic effect, different approaches are used in HIF-1 therapies: HIF-1 upregulation (ischaemia) to induce angiogenesis or HIF-1 inhibition to attenuate angiogenesis (cancer). DMOG: dimethyloxalylglycine; DFO: 2-OG analogue (N-oxalylglycine); PR39: macrophage-derived peptide; AdHIF-1α and AdHIF-2α: adenoviral vector; AdCA5: adenovirus encoding a constitutively active form of the HIF-1α; BMDAC: bone marrow-derived angiogenic cells; HIF-1α/VP16: DNA-binding and dimerisation domains of HIF-1α fused with the transactivation domain of herpes simplex virus; K14-HIF-1ΔODD: vector encoding O2-independent HIF-1α under the keratin 14 promoter. Silibinin: nontoxic flavonoid; topotecan: chemotherapeutic agent that is a topoisomerase inhibitor; SAHA and FK228: histone deacetylase inhibitors; acriflavine, bortezomib; amphotericin B: anti-HIF drugs.
4.1. Activation of HIF-1-Dependent Angiogenesis in Ischaemic Diseases

The neovascularisation of ischaemic regions is a fundamental assumption of therapeutic angiogenesis. The induction of capillary outgrowth for therapeutic purposes is stimulated by the administration of angiogenic growth factors or sequences that encode these proteins. Thus far, multiple angiogenic factors such as VEGF, PlGF, FGF, and PDGF have been applied in in vitro studies and in preclinical and clinical trials [111114]. However, therapies using only one proangiogenic agent to initiate angiogenesis were shown to be insufficient; thus, these therapies may require supplementation with other factors that can stabilise new capillaries. Therefore, hypoxia-induced angiogenesis may be a successful strategy [115]. HIF-1 regulates cell-type specific proangiogenic factors and cytokines either directly or indirectly. Promoting HIF-1 activity is essential in ischaemic diseases. Thus far, HIF-1 therapies have been based on two approaches: the administration of HIF-1α/2α or the induction of HIF-1 expression by the modification/administration/inhibition of molecules associated with HIF activity.

Basic strategies of therapeutic angiogenesis involve the administration of proangiogenic factors using vectors, combined therapies with stem cells or fusion/recombinant proteins. Hypoxia-inducible gene transfer led to the enhancement of angiogenesis in both myocardium and ischaemic skeletal muscles. Additionally, AdHIF-1α and AdHIF-2α injection did not induce tissue oedema in contrast to regions treated with AdVEGF. In conclusion, HIF application in this case resulted in the formation of stable and mature vessels compared to VEGF treatment, where the newly formed vessels were leaky [91]. To prevent oxygen-dependent degradation of HIF-1α, attempts to modify this subunit were made. An alternative approach of applying AdCA5 adenovirus encoding a constitutively active form of the HIF-1α subunit due to a deletion and a point mutation in the region responsible for O2-dependent degradation was proposed. In a model of endovascular limb ischaemia, intramuscular injection of AdCA5 improved the recovery of blood flow by stimulating both angiogenesis and arteriogenesis [116]. Furthermore, therapy with AdCA5 induced the upregulation of several proangiogenic genes/proteins that are targets for therapeutic angiogenesis in hindlimb and cardiac ischaemia models, including FGF-2, hepatocyte growth factor, MCP-1, PDGF-B, PlGF, SDF-1, and VEGF. Moreover, AdCA5 injection into mouse eyes enhanced neovascularisation in multiple capillary beds, including those not responsive to VEGF alone. Due to the upregulation of PlGF and VEGF expression after AdCA5 treatment, both genes acted synergistically, which led to neovascularisation of the retina [117]. Combined therapy using AdCA5 gene therapy and prolyl-4-hydroxylase inhibitor dimethyloxalylglycine- (DMOG-) treated BMDACs (bone marrow-derived angiogenic cells) acted synergistically to increase the recovery of blood flow after femoral artery ligation, thereby preventing tissue necrosis. This synergistic effect is due to AdCA5 enhancement of BMDAC homing, whereas DMOG treatment increases the retention of grafted cells in the ischaemic tissues. Another combined therapy with modified stem cells was applied in cerebral ischaemia. Rat bone marrow-derived mesenchymal stem cells (BMSCs) were infected with adenoviral particles containing constitutively expressed HIF-1α due to mutations in proline 564 and asparagine 803 sites. Genetically modified cells were transplanted in a rat middle cerebral artery occlusion model (MCAO). At 7 days after intervention, improved motor function, reduced cerebral infarction, and increased VEGF protein expression that led to revascularisation were observed [118]. The stabilisation and administration of HIF-1α may be achieved by the construction of a fusion protein. For instance, DNA-binding and dimerisation domains of HIF-1α were fused with the transactivation domain of herpes simplex virus (VP16). A plasmid vector encoding this structure was used in a rabbit hindlimb ischaemia model. The administration of HIF-1α/VP16 resulted in the improvement of blood flow in the ischaemic region as determined by an increase in the number of blood vessels [119]. Additionally, using promoters specific for particular cells or tissues, angiogenesis could have occurred in the designated site. The construction of a vector encoding O2-independent HIF-1α under the keratin 14 promoter (K14-HIF-1ΔODD) resulted in the upregulation of HIF-1α in a skin. The activation of HIF-1α increased skin capillary density. Moreover, the new vessels were not leaky, and enhanced skin vascularity was not associated with oedema compared to the capillaries that formed after K14-VEGF therapy [120].

Another approach leading to HIF-1-dependent angiogenesis is associated with the modification/administration of molecules that regulate HIF-1α activity. The stabilisation of HIF-1α under normoxic conditions is focused on the inhibition of prolyl hydroxylase activity. As mentioned above, PHDs require O2, Fe(II), and 2-oxoglutarate (2-OG) for their enzymatic activity [50, 51]. The delivery of small molecules such as an iron chelator (DFO) or 2-OG analogue (N-oxalylglycine) may inhibit the enzyme activity [121, 122]. For instance, HIF-1 can be enhanced by the suppression of prolyl hydroxylase activity using dimethyloxalylglycine (DMOG). The systemic administration of DMOG in a mouse model of hindlimb ischaemia results in the elevation of HIF-1α protein expression, which leads to neovascularisation in the infarcted region [121]. Then, capillary growth is followed by HIF-induced VEGF and Flk-1 upregulation. A novel strategy involves the preconditioning of rat bone marrow-derived mesenchymal stem cells (BMSCs) using DMOG to enhance their survival and therapeutic efficacy after transplantation into infarcted rat hearts. After DMOG treatment, these cells exhibited enhanced expression of survival and proangiogenic factors such as HIF-1α, vascular endothelial growth factor, and glucose transporter 1. Thus, the transplantation of DMOG-treated BMSCs reduced heart infarction size and promoted angiogenesis in the ischaemic region [123]. An alternative to the use of PHD inhibitor molecules is the administration of a molecule that stabilises HIF-1α under normoxic conditions. PR39, which is a macrophage-derived peptide, achieved this effect by inhibiting HIF-1α degradation through the ubiquitin-proteasome system. The introduction of the PR39 peptide resulted in a significant increase in HIF-1α protein levels irrespective of ischaemia or hypoxia. Therefore, angiogenesis can be induced in the postinfarcted myocardium of transgenic mice [124].

4.2. Inhibition of HIF-1-Dependent Angiogenesis in Cancer Therapies

Cancer therapies are based on the targeted inhibition of proangiogenic factors. Current treatments are focused on the inhibition of VEGF activity. Because of studies regarding VEGF antiangiogenic therapy, patients were treated with bevacizumab (VEGF inhibitor) or sunitinib (VEGFR2 inhibitor) [125]. Since the discovery that the HIF-1 pathway regulates the activation of many proangiogenic factors in tumours and may promote metastasis, the HIF-1α subunit has been considered an attractive target for new cancer therapeutics. The inhibition of HIF-1-dependent angiogenesis involves the regulation of HIF-1α activity by molecules that modulate HIF-1α transcription and transcriptional activity, HIF-1α and HIF-1β dimerisation, HIF-1α protein translation, HIF-1α DNA binding, and HIF-1α protein degradation [24, 126].

Thus far, the Food and Drug Administration has approved several anti-HIF drugs. For instance, bortezomib and amphotericin B functionally inhibit HIF-1α, thereby preventing p300 recruitment by enhancing the interaction between FIH-1 and the HIF-1α C-terminal transactivation domain (C-TAD) [127, 128]. In contrast, silibinin is a nontoxic flavonoid that is able to inhibit hypoxia-dependent HIF-1α accumulation and to inhibit HIF-1 transcriptional activity in HeLa and hepatoma cells [129]. Silibinin is also a potent inhibitor of cell proliferation. Two other drugs, SAHA and FK228, are histone deacetylase inhibitors that were found to promote HIF-1α degradation by upregulating p53 and VHL. These drugs have been successfully used in the United States. HIF-1 activity might also be attenuated by previously used chemotherapeutics such as anthracycline and doxorubicin, which act by inhibiting HIF-1α DNA-binding activity, and acriflavine, which prevents HIF subunit dimerisation [130, 131].

Clinical trials are presently focused on other drugs that may attenuate HIF-1-dependent angiogenesis. Currently, flavopiridol (Alvocidib) is in phase III clinical trials. This synthetic flavonoid, which is derived from an alkaloid Indian plant, was found to be a potent inhibitor of the transcription of HIF-1α and of many other genes involved in cell cycle arrest [132, 133]. Other clinical studies (phases I and II) are being conducted with many other drugs that involve HIF-1 degradation, downregulation, or inactivation.

Conflict of Interests

The authors declare that no conflict of interests exists regarding the publication of this paper.

Acknowledgments

This study was supported by the National Science Centre Grants nos. 2012/07/N/NZ3/01687 and 2014/13/B/NZ3/04646 and by the National Centre for Research and Development Grant no. STRATEGMED1/233624/5/NCBR/2014.

References

  1. G. L. Semenza, “Hypoxia-inducible factor 1 and cardiovascular disease,” Annual Review of Physiology, vol. 76, pp. 39–56, 2014. View at Publisher · View at Google Scholar · View at Scopus
  2. P. Carmeliet, “Mechanisms of angiogenesis and arteriogenesis,” Nature Medicine, vol. 6, no. 4, pp. 389–395, 2000. View at Publisher · View at Google Scholar · View at Scopus
  3. W. Risau, “Mechanisms of angiogenesis,” Nature, vol. 386, no. 6626, pp. 671–674, 1997. View at Publisher · View at Google Scholar · View at Scopus
  4. W. Risau and I. Flamme, “Vasculogenesis,” Annual Review of Cell and Developmental Biology, vol. 11, pp. 73–91, 1995. View at Publisher · View at Google Scholar · View at Scopus
  5. P. Carmeliet, “Angiogenesis in health and disease,” Nature Medicine, vol. 9, no. 6, pp. 653–660, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. G. D. Yancopoulos, S. Davis, N. W. Gale, J. S. Rudge, S. J. Wiegand, and J. Holash, “Vascular-specific growth factors and blood vessel formation,” Nature, vol. 407, no. 6801, pp. 242–248, 2000. View at Publisher · View at Google Scholar · View at Scopus
  7. B. P. Eliceiri, R. Paul, P. L. Schwartzberg, J. D. Hood, J. Leng, and D. A. Cheresh, “Selective requirement for Src kinases during VEGF-induced angiogenesis and vascular permeability,” Molecular Cell, vol. 4, no. 6, pp. 915–924, 1999. View at Publisher · View at Google Scholar · View at Scopus
  8. G. Thurston, J. S. Rudge, E. Ioffe et al., “Angiopoietin-1 protects the adult vasculature against plasma leakage,” Nature Medicine, vol. 6, no. 4, pp. 460–463, 2000. View at Google Scholar
  9. P. C. Maisonpierre, C. Suri, P. F. Jones et al., “Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis,” Science, vol. 277, no. 5322, pp. 55–60, 1997. View at Publisher · View at Google Scholar · View at Scopus
  10. A. R. Nelson, B. Fingleton, M. L. Rothenberg, and L. M. Matrisian, “Matrix metalloproteinases: biologic activity and clinical implications,” Journal of Clinical Oncology, vol. 18, no. 5, pp. 1135–1149, 2000. View at Google Scholar · View at Scopus
  11. E. M. Conway, D. Collen, and P. Carmeliet, “Molecular mechanisms of blood vessel growth,” Cardiovascular Research, vol. 49, no. 3, pp. 507–521, 2001. View at Publisher · View at Google Scholar · View at Scopus
  12. B. P. Eliceiri and D. A. Cheresh, “The role of αv integrins during angiogenesis: insights into potential mechanisms of action and clinical development,” Journal of Clinical Investigation, vol. 103, no. 9, pp. 1227–1230, 1999. View at Publisher · View at Google Scholar · View at Scopus
  13. M. Corada, M. Mariotti, G. Thurston et al., “Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 17, pp. 9815–9820, 1999. View at Publisher · View at Google Scholar · View at Scopus
  14. K. A. Knudsen, C. Frankowski, K. R. Johnson, and M. J. Wheelock, “A role for cadherins in cellular signaling and differentiation,” Journal of Cellular Biochemistry, vol. 30-31, pp. 168–176, 1998. View at Google Scholar · View at Scopus
  15. P. Lindahl, M. Hellström, M. Kalén, and C. Betsholtz, “Endothelial-perivascular cell signaling in vascular development: lessons from knockout mice,” Current Opinion in Lipidology, vol. 9, no. 5, pp. 407–411, 1998. View at Publisher · View at Google Scholar · View at Scopus
  16. P. J. Polverini, “The pathophysiology of angiogenesis,” Critical Reviews in Oral Biology and Medicine, vol. 6, no. 3, pp. 230–247, 1995. View at Publisher · View at Google Scholar · View at Scopus
  17. A. S. Chung, J. Lee, and N. Ferrara, “Targeting the tumour vasculature: insights from physiological angiogenesis,” Nature Reviews Cancer, vol. 10, no. 7, pp. 505–514, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. R. S. Kerbel, “Tumor angiogenesis,” The New England Journal of Medicine, vol. 358, no. 19, pp. 2039–2049, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. N. Ferrara and R. S. Kerbel, “Angiogenesis as a therapeutic target,” Nature, vol. 438, no. 7070, pp. 967–974, 2005. View at Publisher · View at Google Scholar · View at Scopus
  20. R. K. Jain, “Molecular regulation of vessel maturation,” Nature Medicine, vol. 9, no. 6, pp. 685–693, 2003. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Nagy, S.-H. Chang, A. M. Dvorak, and H. F. Dvorak, “Why are tumour blood vessels abnormal and why is it important to know?” British Journal of Cancer, vol. 100, no. 6, pp. 865–869, 2009. View at Publisher · View at Google Scholar · View at Scopus
  22. G. L. Semenza, “Life with oxygen,” Science, vol. 318, no. 5847, pp. 62–64, 2007. View at Publisher · View at Google Scholar · View at Scopus
  23. J. A. Bertout, S. A. Patel, and M. C. Simon, “The impact of O2 availability on human cancer,” Nature Reviews Cancer, vol. 8, no. 12, pp. 967–975, 2008. View at Publisher · View at Google Scholar · View at Scopus
  24. B. L. Krock, N. Skuli, and M. C. Simon, “Hypoxia-induced angiogenesis: good and evil,” Genes and Cancer, vol. 2, no. 12, pp. 1117–1133, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. Z. Yun, Q. Lin, and A. J. Giaccia, “Adaptive myogenesis under hypoxia,” Molecular and Cellular Biology, vol. 25, no. 8, pp. 3040–3055, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. J. I. Bárdos and M. Ashcroft, “Negative and positive regulation of HIF-1: a complex network,” Biochimica et Biophysica Acta—Reviews on Cancer, vol. 1755, no. 2, pp. 107–120, 2005. View at Publisher · View at Google Scholar · View at Scopus
  27. G. L. Semenza and G. L. Wang, “A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation,” Molecular and Cellular Biology, vol. 12, no. 12, pp. 5447–5454, 1992. View at Google Scholar · View at Scopus
  28. G. L. Wang and G. L. Semenza, “Characterization of hypoxia-inducible factor 1 and regulation of DNA binding activity by hypoxia,” The Journal of Biological Chemistry, vol. 268, no. 29, pp. 21513–21518, 1993. View at Google Scholar · View at Scopus
  29. R. J. Kewley, M. L. Whitelaw, and A. Chapman-Smith, “The mammalian basic helix-loop-helix/PAS family of transcriptional regulators,” International Journal of Biochemistry and Cell Biology, vol. 36, no. 2, pp. 189–204, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. G. L. Wang, B.-H. Jiang, E. A. Rue, and G. L. Semenza, “Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 12, pp. 5510–5514, 1995. View at Publisher · View at Google Scholar · View at Scopus
  31. S. T. Crews, “Control of cell lineage-specific development and transcription by bHLH-PAS proteins,” Genes and Development, vol. 12, no. 5, pp. 607–620, 1998. View at Publisher · View at Google Scholar · View at Scopus
  32. R. H. Wenger, D. P. Stiehl, and G. Camenisch, “Integration of oxygen signaling at the consensus HRE,” Science's STKE, vol. 2005, no. 306, article re12, 2005. View at Google Scholar · View at Scopus
  33. J. L. Ruas, L. Poellinger, and T. Pereira, “Functional analysis of hypoxia-inducible factor-1 alpha mediated transactivation. Identification of amino acid residues critical for transcriptional activation and/or interaction with CREB-binding protein,” The Journal of Biological Chemistry, vol. 277, no. 41, pp. 38723–38730, 2002. View at Publisher · View at Google Scholar · View at Scopus
  34. B.-H. Jiang, J. Z. Zheng, S. W. Leung, R. Roe, and G. L. Semenza, “Transactivation and inhibitory domains of hypoxia-inducible factor 1α: modulation of transcriptional activity by oxygen tension,” The Journal of Biological Chemistry, vol. 272, no. 31, pp. 19253–19260, 1997. View at Publisher · View at Google Scholar · View at Scopus
  35. C. W. Pugh, J. F. O'Rourke, M. Nagao, J. M. Gleadle, and P. J. Ratcliffe, “Activation of hypoxia-inducible factor-1: definition of regulatory domains within the α subunit,” The Journal of Biological Chemistry, vol. 272, no. 17, pp. 11205–11214, 1997. View at Publisher · View at Google Scholar · View at Scopus
  36. B.-H. Jiang, G. L. Semenza, C. Bauer, and H. H. Marti, “Hypoxia-inducible factor 1 levels vary exponentially over a physiologically relevant range of O2 tension,” The American Journal of Physiology—Cell Physiology, vol. 271, no. 4, pp. C1172–C1180, 1996. View at Google Scholar · View at Scopus
  37. S. Salceda and J. Caro, “Hypoxia-inducible factor 1α (HIF-1α) protein is rapidly degraded by the ubiquitin-proteasome system under normoxic conditions. Its stabilization by hypoxia depends on redox-induced changes,” Journal of Biological Chemistry, vol. 272, no. 36, pp. 22642–22647, 1997. View at Publisher · View at Google Scholar · View at Scopus
  38. M. Ema, S. Taya, N. Yokotani, K. Sogawa, Y. Matsuda, and Y. Fujii-Kuriyama, “A novel bHLH-PAS factor with close sequence similarity to hypoxia-inducible factor 1α regulates the VEGF expression and is potentially involved in lung and vascular development,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 9, pp. 4273–4278, 1997. View at Publisher · View at Google Scholar · View at Scopus
  39. Y.-Z. Gu, S. M. Moran, J. B. Hogenesch, L. Wartman, and C. A. Bradfield, “Molecular characterization and chromosomal localization of a third α-class hypoxia inducible factor subunit, HIF3α,” Gene Expression, vol. 7, no. 3, pp. 205–213, 1998. View at Google Scholar · View at Scopus
  40. Y. Makino, R. Cao, K. Svensson et al., “Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression,” Nature, vol. 414, no. 6863, pp. 550–554, 2001. View at Publisher · View at Google Scholar · View at Scopus
  41. W. G. Kaelin Jr., “Proline hydroxylation and gene expression,” Annual Review of Biochemistry, vol. 74, pp. 115–128, 2005. View at Publisher · View at Google Scholar · View at Scopus
  42. V. Srinivas, L.-P. Zhang, X.-H. Zhu, and J. Caro, “Characterization of an oxygen/redox-dependent degradation domain of hypoxia-inducible factor α (HIF-α) proteins,” Biochemical and Biophysical Research Communications, vol. 260, no. 2, pp. 557–561, 1999. View at Publisher · View at Google Scholar · View at Scopus
  43. N. Masson and P. J. Ratcliffe, “HIF prolyl and asparaginyl hydroxylases in the biological response to intracellular O2 levels,” Journal of Cell Science, vol. 116, no. 15, pp. 3041–3049, 2003. View at Publisher · View at Google Scholar · View at Scopus
  44. N. Masson, C. Willam, P. H. Maxwell, C. W. Pugh, and P. J. Ratcliffe, “Independent function of two destruction domains in hypoxia-inducible factor-α chains activated by prolyl hydroxylation,” EMBO Journal, vol. 20, no. 18, pp. 5197–5206, 2001. View at Publisher · View at Google Scholar · View at Scopus
  45. L. A. McNeill, K. S. Hewitson, J. M. Gleadle et al., “The use of dioxygen by HIF prolyl hydroxylase (PHD1),” Bioorganic & Medicinal Chemistry Letters, vol. 12, no. 12, pp. 1547–1550, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. K. S. Hewitson, L. A. McNeill, M. V. Riordan et al., “Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical to factor inhibiting HIF (FIH) and is related to the cupin structural family,” The Journal of Biological Chemistry, vol. 277, no. 29, pp. 26351–26355, 2002. View at Publisher · View at Google Scholar · View at Scopus
  47. D. Lando, D. J. Peet, D. A. Whelan, J. J. Gorman, and M. L. Whitelaw, “Asparagine hydroxylation of the HIF transactivation domain: a hypoxic switch,” Science, vol. 295, no. 5556, pp. 858–861, 2002. View at Publisher · View at Google Scholar · View at Scopus
  48. D. Lando, D. J. Peet, J. J. Gorman, D. A. Whelan, M. L. Whitelaw, and R. K. Bruick, “FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor,” Genes and Development, vol. 16, no. 12, pp. 1466–1471, 2002. View at Publisher · View at Google Scholar · View at Scopus
  49. P. C. Mahon, K. Hirota, and G. L. Semenza, “FIH-1: a novel protein that interacts with HIF-1α and VHL to mediate repression of HIF-1 transcriptional activity,” Genes and Development, vol. 15, no. 20, pp. 2675–2686, 2001. View at Publisher · View at Google Scholar · View at Scopus
  50. W. G. Kaelin Jr. and P. J. Ratcliffe, “Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway,” Molecular Cell, vol. 30, no. 4, pp. 393–402, 2008. View at Publisher · View at Google Scholar · View at Scopus
  51. C. J. Schofield and Z. Zhang, “Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes,” Current Opinion in Structural Biology, vol. 9, no. 6, pp. 722–731, 1999. View at Publisher · View at Google Scholar · View at Scopus
  52. Q. Ke and M. Costa, “Hypoxia-inducible factor-1 (HIF-1),” Molecular Pharmacology, vol. 70, no. 5, pp. 1469–1480, 2006. View at Publisher · View at Google Scholar · View at Scopus
  53. D. J. Manalo, A. Rowan, T. Lavoie et al., “Transcriptional regulation of vascular endothelial cell responses to hypoxia by HIF-1,” Blood, vol. 105, no. 2, pp. 659–669, 2005. View at Publisher · View at Google Scholar · View at Scopus
  54. P. J. Kallio, I. Pongratz, K. Gradin, J. McGuire, and L. Poellinger, “Activation of hypoxia-inducible factor 1α: posttranscriptional regulation and conformational change by recruitment of the Arnt transcription factor,” Proceedings of the National Academy of Sciences of the United States of America, vol. 94, no. 11, pp. 5667–5672, 1997. View at Publisher · View at Google Scholar · View at Scopus
  55. P. J. Kallio, K. Okamoto, S. O'Brien et al., “Signal transduction in hypoxic cells: inducible nuclear translocation and recruitment of the CBP/p300 coactivator by the hypoxia-inducible factor-1α,” The EMBO Journal, vol. 17, no. 22, pp. 6573–6586, 1998. View at Publisher · View at Google Scholar · View at Scopus
  56. G. L. Semenza, M. K. Nejfelt, S. M. Chi, and S. E. Antonarakis, “Hypoxia-inducible nuclear factors bind to an enhancer element located 3′ to the human erythropoietin gene,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 13, pp. 5680–5684, 1991. View at Publisher · View at Google Scholar · View at Scopus
  57. T. N. Seagroves, H. E. Ryan, H. Lu et al., “Transcription factor HIF-1 is a necessary mediator of the pasteur effect in mammalian cells,” Molecular and Cellular Biology, vol. 21, no. 10, pp. 3436–3444, 2001. View at Publisher · View at Google Scholar · View at Scopus
  58. C. V. Dang and G. L. Semenza, “Oncogenic alterations of metabolism,” Trends in Biochemical Sciences, vol. 24, no. 2, pp. 68–72, 1999. View at Publisher · View at Google Scholar · View at Scopus
  59. G. L. Semenza, P. H. Roth, H.-M. Fang, and G. L. Wang, “Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1,” The Journal of Biological Chemistry, vol. 269, no. 38, pp. 23757–23763, 1994. View at Google Scholar · View at Scopus
  60. R. H. Wenger, “Cellular adaptation to hypoxia: O2-sensing protein hydroxylases, hypoxia-inducible transcription factors, and O2-regulated gene expression,” The FASEB Journal, vol. 16, no. 10, pp. 1151–1162, 2002. View at Publisher · View at Google Scholar · View at Scopus
  61. G. L. Semenza, “Regulation of metabolism by hypoxia-inducible factor 1,” Cold Spring Harbor Symposia on Quantitative Biology, vol. 76, pp. 347–353, 2011. View at Publisher · View at Google Scholar · View at Scopus
  62. D. Feldser, F. Agani, N. V. Iyer, B. Pak, G. Ferreira, and G. L. Semenza, “Reciprocal positive regulation of hypoxia-inducible factor 1alpha and insulin-like growth factor 2,” Cancer Research, vol. 59, no. 16, pp. 3915–3918, 1999. View at Google Scholar · View at Scopus
  63. B. Krishnamachary, S. Berg-Dixon, B. Kelly et al., “Regulation of colon carcinoma cell invasion by hypoxia-inducible factor 1,” Cancer Research, vol. 63, no. 5, pp. 1138–1143, 2003. View at Google Scholar · View at Scopus
  64. M. Koning, P. M. N. Werker, M. J. A. van Luyn, and M. C. Harmsen, “Hypoxia promotes proliferation of human myogenic satellite cells: a potential benefactor in tissue engineering of skeletal muscle,” Tissue Engineering A, vol. 17, no. 13-14, pp. 1747–1758, 2011. View at Publisher · View at Google Scholar · View at Scopus
  65. J. A. Mitchell and J. M. Yochim, “Intrauterine oxygen tension during the estrous cycle in the rat: its relation to uterine respiration and vascular activity,” Endocrinology, vol. 83, no. 4, pp. 701–705, 1968. View at Publisher · View at Google Scholar · View at Scopus
  66. F. Rodesch, P. Simon, C. Donner, and E. Jauniaux, “Oxygen measurements in endometrial and trophoblastic tissues during early pregnancy,” Obstetrics and Gynecology, vol. 80, no. 2, pp. 283–285, 1992. View at Google Scholar · View at Scopus
  67. H. Diez, A. Fischer, A. Winkler et al., “Hypoxia-mediated activation of Dll4-Notch-Hey2 signaling in endothelial progenitor cells and adoption of arterial cell fate,” Experimental Cell Research, vol. 313, no. 1, pp. 1–9, 2007. View at Publisher · View at Google Scholar · View at Scopus
  68. D. M. Adelman, E. Maltepe, and M. C. Simon, “Multilineage embryonic hematopoiesis requires hypoxic ARNT activity,” Genes and Development, vol. 13, no. 19, pp. 2478–2483, 1999. View at Publisher · View at Google Scholar · View at Scopus
  69. N. V. Iyer, L. E. Kotch, F. Agani et al., “Cellular and developmental control of O2 homeostasis by hypoxia-inducible factor 1α,” Genes & Development, vol. 12, no. 2, pp. 149–162, 1998. View at Publisher · View at Google Scholar · View at Scopus
  70. K. R. Kozak, B. Abbott, and O. Hankinson, “ARNT-deficient mice and placental differentiation,” Developmental Biology, vol. 191, no. 2, pp. 297–305, 1997. View at Publisher · View at Google Scholar · View at Scopus
  71. D. M. Adelman, M. Gertsenstein, A. Nagy, M. C. Simon, and E. Maltepe, “Placental cell fates are regulated in vivo by HIF-mediated hypoxia responses,” Genes and Development, vol. 14, no. 24, pp. 3191–3203, 2000. View at Publisher · View at Google Scholar · View at Scopus
  72. K. D. Cowden Dahl, B. H. Fryer, F. A. Mack et al., “Hypoxia-inducible factors 1α and 2α regulate trophoblast differentiation,” Molecular and Cellular Biology, vol. 25, no. 23, pp. 10479–10491, 2005. View at Publisher · View at Google Scholar · View at Scopus
  73. K. Takeda, V. C. Ho, H. Takeda, L. J. Duan, A. Nagy, and G.-H. Fong, “Placental but not heart defects are associated with elevated hypoxia-inducible factor α levels in mice lacking prolyl hydroxylase domain protein 2,” Molecular and Cellular Biology, vol. 26, no. 22, pp. 8336–8346, 2006. View at Publisher · View at Google Scholar · View at Scopus
  74. E. Maltepe, J. V. Schmidt, D. Baunoch, C. A. Bradfield, and M. C. Simon, “Abnormal angiogenesis and responses to glucose and oxygen deprivation in mice lacking the protein ARNT,” Nature, vol. 386, no. 6623, pp. 403–407, 1997. View at Publisher · View at Google Scholar · View at Scopus
  75. D. L. Ramírez-Bergeron, A. Runge, D. M. Adelman, M. Gohil, and M. C. Simon, “HIF-dependent hematopoietic factors regulate the development of the embryonic vasculature,” Developmental Cell, vol. 11, no. 1, pp. 81–92, 2006. View at Publisher · View at Google Scholar · View at Scopus
  76. A. E. Greijer, P. van der Groep, D. Kemming et al., “Up-regualtion of gene expression by hypoxia is mediated predominantly by hypoxia-inducible factor I (HIF-I),” Journal of Pathology, vol. 206, no. 3, pp. 291–304, 2005. View at Publisher · View at Google Scholar · View at Scopus
  77. G. L. Semenza, “Angiogenesis in ischemic and neoplastic disorders,” Annual Review of Medicine, vol. 54, pp. 17–28, 2003. View at Publisher · View at Google Scholar · View at Scopus
  78. D. J. Ceradini, A. R. Kulkarni, M. J. Callaghan et al., “Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1,” Nature Medicine, vol. 10, no. 8, pp. 858–864, 2004. View at Publisher · View at Google Scholar · View at Scopus
  79. J. E. Park, G.-A. Keller, and N. Ferrara, “The vascular endothelial growth factor (VEGF) isoforms: differential deposition into the subepithelial extracellular matrix and bioactivity of extracellular matrix-bound VEGF,” Molecular Biology of the Cell, vol. 4, no. 12, pp. 1317–1326, 1993. View at Publisher · View at Google Scholar · View at Scopus
  80. Y. Liu, S. R. Cox, T. Morita, and S. Kourembanas, “Hypoxia regulates vascular endothelial growth factor gene expression in endothelial cells: Identification of a 5′ enhancer,” Circulation Research, vol. 77, no. 3, pp. 638–643, 1995. View at Publisher · View at Google Scholar · View at Scopus
  81. J. A. Forsythe, B.-H. Jiang, N. V. Iyer et al., “Activation of vascular endothelial growth factor gene transcription by hypoxia-inducible factor 1,” Molecular and Cellular Biology, vol. 16, no. 9, pp. 4604–4613, 1996. View at Google Scholar · View at Scopus
  82. H.-P. Gerber, F. Condorelli, J. Park, and N. Ferrara, “Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. Flt-1, but not Flk-1/KDR, is up-regulated by hypoxia,” The Journal of Biological Chemistry, vol. 272, no. 38, pp. 23659–23667, 1997. View at Publisher · View at Google Scholar · View at Scopus
  83. J. Waltenberger, U. Mayr, S. Pentz, and V. Hombach, “Functional upregulation of the vascular endothelial growth factor receptor KDR by hypoxia,” Circulation, vol. 94, no. 7, pp. 1647–1654, 1996. View at Publisher · View at Google Scholar · View at Scopus
  84. C. W. Pugh and P. J. Ratcliffe, “Regulation of angiogenesis by hypoxia: role of the HIF system,” Nature Medicine, vol. 9, no. 6, pp. 677–684, 2003. View at Publisher · View at Google Scholar · View at Scopus
  85. D. R. Mole, C. Blancher, R. R. Copley et al., “Genome-wide association of hypoxia-inducible factor (HIF)-1α and HIF-2α DNA binding with expression profiling of hypoxia-inducible transcripts,” The Journal of Biological Chemistry, vol. 284, no. 25, pp. 16767–16775, 2009. View at Publisher · View at Google Scholar · View at Scopus
  86. J. Schödel, S. Oikonomopoulos, J. Ragoussis, C. W. Pugh, P. J. Ratcliffe, and D. R. Mole, “High-resolution genome-wide mapping of HIF-binding sites by ChIP-seq,” Blood, vol. 117, no. 23, pp. e207–e217, 2011. View at Publisher · View at Google Scholar · View at Scopus
  87. P. Carmeliet and R. K. Jain, “Molecular mechanisms and clinical applications of angiogenesis,” Nature, vol. 473, no. 7347, pp. 298–307, 2011. View at Publisher · View at Google Scholar · View at Scopus
  88. Y. Ben-Yosef, A. Miller, S. Shapiro, and N. Lahat, “Hypoxia of endothelial cells leads to MMP-2-dependent survival and death,” The American Journal of Physiology—Cell Physiology, vol. 289, no. 5, pp. C1321–C1331, 2005. View at Publisher · View at Google Scholar · View at Scopus
  89. S. Keely, L. E. Glover, C. F. MacManus et al., “Selective induction of integrin β1 by hypoxia-inducible factor: implications for wound healing,” The FASEB Journal, vol. 23, no. 5, pp. 1338–1346, 2009. View at Publisher · View at Google Scholar · View at Scopus
  90. N. Skuli, L. Liu, A. Runge et al., “Endothelial deletion of hypoxia-inducible factor-2α (HIF-2α) alters vascular function and tumor angiogenesis,” Blood, vol. 114, no. 2, pp. 469–477, 2009. View at Publisher · View at Google Scholar · View at Scopus
  91. H. Niemi, K. Honkonen, P. Korpisalo et al., “HIF-1α and HIF-2α induce angiogenesis and improve muscle energy recovery,” European Journal of Clinical Investigation, vol. 44, no. 10, pp. 989–999, 2014. View at Publisher · View at Google Scholar
  92. C. Yuan, P. Wang, L. Zhu et al., “Coculture of stem cells from apical papilla and human umbilical vein endothelial cell under hypoxia increases the formation of three-dimensional vessel-like structures in vitro,” Tissue Engineering Part A, vol. 21, no. 5-6, 2015. View at Google Scholar
  93. S. Rajagopalan, J. Olin, S. Deitcher et al., “Use of a constitutively active hypoxia-inducible factor-1α transgene as a therapeutic strategy in no-option critical limb ischemia patients: phase I dose-escalation experience,” Circulation, vol. 115, no. 10, pp. 1234–1243, 2007. View at Publisher · View at Google Scholar · View at Scopus
  94. L. Holmgren, M. S. O'Reilly, and J. Folkman, “Dormancy of micrometastases: balanced proliferation and apoptosis in the presence of angiogenesis suppression,” Nature Medicine, vol. 1, no. 2, pp. 149–153, 1995. View at Publisher · View at Google Scholar · View at Scopus
  95. J. Folkman, “Tumour angiogenesis,” in Cancer Medicine, J. F. Holland, R. C. Bast Jr., D. L. Morton, E. Frei III, D. W. Kufe, and R. R. Weichselbaum, Eds., pp. 181–204, Williams & Wilkins, Baltimore, Md, USA, 1997. View at Google Scholar
  96. E. B. Rankin and A. J. Giaccia, “The role of hypoxia-inducible factors in tumorigenesis,” Cell Death and Differentiation, vol. 15, no. 4, pp. 678–685, 2008. View at Publisher · View at Google Scholar · View at Scopus
  97. J. M. Brown and A. J. Giaccia, “The unique physiology of solid tumours: opportunities (and problems) for cancer therapy,” Cancer Research, vol. 58, no. 7, pp. 1408–1416, 1998. View at Google Scholar · View at Scopus
  98. G. L. Semenza, “Hypoxia, clonal selection, and the role of HIF-1 in tumor progression,” Critical Reviews in Biochemistry and Molecular Biology, vol. 35, no. 2, pp. 71–103, 2000. View at Publisher · View at Google Scholar · View at Scopus
  99. R. Ravi, B. Mookerjee, Z. M. Bhujwalla et al., “Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor 1α,” Genes and Development, vol. 14, no. 1, pp. 34–44, 2000. View at Google Scholar · View at Scopus
  100. W. Zundel, C. Schindler, D. Haas-Kogan et al., “Loss of PTEN facilitates HIF-1-mediated gene expression,” Genes and Development, vol. 14, no. 4, pp. 391–396, 2000. View at Google Scholar · View at Scopus
  101. E. Laughner, P. Taghavi, K. Chiles, P. C. Mahon, and G. L. Semenza, “HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1α (HIF-1α) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression,” Molecular and Cellular Biology, vol. 21, no. 12, pp. 3995–4004, 2001. View at Publisher · View at Google Scholar · View at Scopus
  102. W. G. Kaelin Jr., “Molecular basis of the VHL hereditary cancer syndrome,” Nature Reviews Cancer, vol. 2, no. 9, pp. 673–682, 2002. View at Publisher · View at Google Scholar · View at Scopus
  103. P. H. Maxwell, M. S. Wlesener, G. W. Chang et al., “The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis,” Nature, vol. 399, no. 6733, pp. 271–275, 1999. View at Publisher · View at Google Scholar · View at Scopus
  104. O. Iliopoulos, A. Kibel, S. Gray, and W. G. Kaelin Jr., “Tumour suppression by the human von Hippel-Lindau gene product,” Nature Medicine, vol. 1, no. 8, pp. 822–826, 1995. View at Publisher · View at Google Scholar · View at Scopus
  105. K. Kondo, J. Klco, E. Nakamura, M. Lechpammer, and W. G. Kaelin Jr., “Inhibition of HIF is necessary for tumor suppression by the von Hippel-Lindau protein,” Cancer Cell, vol. 1, no. 3, pp. 237–246, 2002. View at Publisher · View at Google Scholar · View at Scopus
  106. J. K. Maranchie, J. R. Vasselli, J. Riss, J. S. Bonifacino, W. M. Linehan, and R. D. Klausner, “The contribution of VHL substrate binding and HIF1-α to the phenotype of VHL loss in renal cell carcinoma,” Cancer Cell, vol. 1, no. 3, pp. 247–255, 2002. View at Publisher · View at Google Scholar · View at Scopus
  107. R. R. Raval, K. W. Lau, M. G. B. Tran et al., “Contrasting properties of hypoxia-inducible factor 1 (HIF-1) and HIF-2 in von Hippel-Lindau-associated renal cell carcinoma,” Molecular and Cellular Biology, vol. 25, no. 13, pp. 5675–5686, 2005. View at Publisher · View at Google Scholar · View at Scopus
  108. H. Lu, R. A. Forbes, and A. Verma, “Hypoxia-inducible factor 1 activation by aerobic glycolysis implicates the Warburg effect in carcinogenesis,” The Journal of Biological Chemistry, vol. 277, no. 26, pp. 23111–23115, 2002. View at Publisher · View at Google Scholar · View at Scopus
  109. Z. Wang, C. Dabrosin, X. Yin et al., “Broad targeting of angiogenesis for cancer prevention and therapy,” Seminars in Cancer Biology, 2015. View at Publisher · View at Google Scholar
  110. P. N. Span and J. Bussink, “Biology of hypoxia,” Seminars in Nuclear Medicine, vol. 45, no. 2, pp. 101–109, 2015. View at Google Scholar
  111. A. Zimna, A. Janeczek, N. Rozwadowska et al., “Biological properties of human skeletal myoblasts genetically modified to simultaneously overexpress the pro-angiogenic factors vascular endothelial growth factor-A and fibroblast growth factor-4,” Journal of Physiology and Pharmacology, vol. 65, no. 2, pp. 193–207, 2014. View at Google Scholar · View at Scopus
  112. S. Kolakowski Jr., M. F. Berry, P. Atluri et al., “Placental growth factor provides a novel local angiogenic therapy for ischemic cardiomyopathy,” Journal of Cardiac Surgery, vol. 21, no. 6, pp. 559–564, 2006. View at Publisher · View at Google Scholar · View at Scopus
  113. S. Rajagopalan, E. R. Mohler III, R. J. Lederman et al., “Regional angiogenesis with vascular endothelial growth factor in peripheral arterial disease: a phase II randomized, double-blind, controlled study of adenoviral delivery of vascular endothelial growth factor 121 in patients with disabling intermittent claudication,” Circulation, vol. 108, no. 16, pp. 1933–1938, 2003. View at Publisher · View at Google Scholar · View at Scopus
  114. R. J. Lederman, F. O. Mendelsohn, R. D. Anderson et al., “TRAFFIC Investigators. Therapeutic angiogenesis with recombinant fibroblast growth factor-2 for intermittent claudication (the TRAFFIC study): a randomised trial,” The Lancet, vol. 359, no. 9323, pp. 2953–2958, 2002. View at Publisher · View at Google Scholar · View at Scopus
  115. K. Hirota and G. L. Semenza, “Regulation of angiogenesis by hypoxia-inducible factor 1,” Critical Reviews in Oncology/Hematology, vol. 59, no. 1, pp. 15–26, 2006. View at Publisher · View at Google Scholar · View at Scopus
  116. T. H. Patel, H. Kimura, C. R. Weiss, G. L. Semenza, and L. V. Hofmann, “Constitutively active HIF-1α improves perfusion and arterial remodeling in an endovascular model of limb ischemia,” Cardiovascular Research, vol. 68, no. 1, pp. 144–154, 2005. View at Publisher · View at Google Scholar · View at Scopus
  117. B. D. Kelly, S. F. Hackett, K. Hirota et al., “Cell type-specific regulation of angiogenic growth factor gene expression and induction of angiogenesis in nonischemic tissue by a constitutively active form of hypoxia-inducible factor 1,” Circulation Research, vol. 93, no. 11, pp. 1074–1081, 2003. View at Publisher · View at Google Scholar · View at Scopus
  118. C. Yang, H. Liu, and D. Liu, “Mutant hypoxia-inducible factor 1α modified bone marrow mesenchymal stem cells ameliorate cerebral ischemia,” International Journal of Molecular Medicine, vol. 34, no. 6, pp. 1622–1628, 2014. View at Publisher · View at Google Scholar
  119. K.-G. Shyu, M.-T. Wang, B.-W. Wang et al., “Intramyocardial injection of naked DNA encoding HIF-1α/VP16 hybrid to enhance angiogenesis in an acute myocardial infarction model in the rat,” Cardiovascular Research, vol. 54, no. 3, pp. 576–583, 2002. View at Publisher · View at Google Scholar · View at Scopus
  120. D. A. Elson, G. Thurston, L. E. Huang et al., “Induction of hypervascularity without leakage or inflammation in transgenic mice overexpressing hypoxia-inducible factor-1α,” Genes and Development, vol. 15, no. 19, pp. 2520–2532, 2001. View at Publisher · View at Google Scholar · View at Scopus
  121. M. Milkiewicz, C. W. Pugh, and S. Egginton, “Inhibition of endogenous HIF inactivation induces angiogenesis in ischaemic skeletal muscles of mice,” Journal of Physiology, vol. 560, no. 1, pp. 21–26, 2004. View at Publisher · View at Google Scholar · View at Scopus
  122. G. L. Wang and G. L. Semenza, “Desferrioxamine induces erythropoietin gene expression and hypoxia-inducible factor 1 DNA-binding activity: Implications for models of hypoxia signal transduction,” Blood, vol. 82, no. 12, pp. 3610–3615, 1993. View at Google Scholar · View at Scopus
  123. X. Liu, J. Wang, X. Ji, S. Yu, and L. Wei, “Preconditioning of bone marrow mesenchymal stem cells by prolyl hydroxylase inhibition enhances cell survival and angiogenesis in vitro and after transplantation into the ischemic heart of rats,” Stem Cell Research & Therapy, vol. 5, no. 5, p. 111, 2014. View at Publisher · View at Google Scholar
  124. J. Li, M. Post, R. Volk et al., “PR39, a peptide regulator of angiogenesis,” Nature Medicine, vol. 61, pp. 49–55, 2000. View at Google Scholar
  125. K. Mittal, H. Koon, P. Elson et al., “Dual VEGF/VEGFR inhibition in advanced solid malignancies,” Cancer Biology & Therapy, vol. 15, no. 8, pp. 975–981, 2014. View at Publisher · View at Google Scholar
  126. Y. Hu, J. Liu, and H. Huang, “Recent agents targeting HIF-1α for cancer therapy,” Journal of Cellular Biochemistry, vol. 114, no. 3, pp. 498–509, 2013. View at Publisher · View at Google Scholar · View at Scopus
  127. D. H. Shin, Y.-S. Chun, D. S. Lee, L. E. Huang, and J.-W. Park, “Bortezomib inhibits tumor adaptation to hypoxia by stimulating the FIH-mediated repression of hypoxia-inducible factor-1,” Blood, vol. 111, no. 6, pp. 3131–3136, 2008. View at Publisher · View at Google Scholar · View at Scopus
  128. E. J. Yeo, Y. S. Chun, Y. S. Cho et al., “YC-1: a potential anticancer drug targeting hypoxia-inducible factor 1,” Journal of the National Cancer Institute, vol. 95, no. 7, pp. 516–525, 2003. View at Publisher · View at Google Scholar · View at Scopus
  129. P. García-Maceira and J. Mateo, “Silibinin inhibits hypoxia-inducible factor-1α and mTOR/p70S6K/4E-BP1 signalling pathway in human cervical and hepatoma cancer cells: implications for anticancer therapy,” Oncogene, vol. 28, no. 3, pp. 313–324, 2009. View at Publisher · View at Google Scholar · View at Scopus
  130. Y. M. Lee, S.-H. Kim, H.-S. Kim et al., “Inhibition of hypoxia-induced angiogenesis by FK228, a specific histone deacetylase inhibitor, via suppression of HIF-1α activity,” Biochemical and Biophysical Research Communications, vol. 300, no. 1, pp. 241–246, 2003. View at Publisher · View at Google Scholar · View at Scopus
  131. S. Shankar, R. Davis, K. P. Singh, R. Kurzrock, D. D. Ross, and R. K. Srivastava, “Suberoylanilide hydroxamic acid (Zolinza/vorinostat) sensitizes TRAIL-resistant breast cancer cells orthotopically implanted in BALB/c nude mice,” Molecular Cancer Therapeutics, vol. 8, no. 6, pp. 1596–1605, 2009. View at Publisher · View at Google Scholar · View at Scopus
  132. E. W. Newcomb, M. A. Ali, T. Schnee et al., “Flavopiridol downregulates hypoxia-mediated hypoxia-inducible factor-1α expression in human glioma cells by a proteasome-independent pathway: implications for in vivo therapy,” Neuro-Oncology, vol. 7, no. 3, pp. 225–235, 2005. View at Publisher · View at Google Scholar · View at Scopus
  133. M. V. Blagosklonny, “Flavopiridol, an inhibitor of transcription: implications, problems and solutions,” Cell Cycle, vol. 3, no. 12, pp. 1537–1542, 2004. View at Publisher · View at Google Scholar · View at Scopus
  134. C. J. Yeom, L. Zeng, Y. Zhu, M. Hiraoka, and H. Harada, “Strategies to assess hypoxic/HIF-1-active cancer cells for the development of innovative radiation therapy,” Cancers, vol. 3, no. 3, pp. 3610–3631, 2011. View at Publisher · View at Google Scholar · View at Scopus