Vitamin E Supplementation Delays Cellular Senescence In Vitro

Figure 3

Vitamin E treatment does not alter the percentage of proliferating HUVECs. (a) Representative images showing passage 5 and passage 9 (CTR and vitamin E treatment) HUVEC cultures stained for the proliferation marker EdU (red). Nuclei are visualized by Hoechst staining (blue). Scale bars = 100 μm. (b) Graphs represent the percentage of EdU positive HUVECs. Error bars represent s.e.m. For all analyses . Passage 5: CTR = 37%, vitamin E = 35%. test: (ns). Passage 9: Ctrl = 27%, vitamin E = 27%. test: (ns).

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