Identification of a New Alcaligenes faecalis Strain MOR02 and Assessment of Its Toxicity and Pathogenicity to Insects

Table 1

Mortality assessment in the pathogenicity assay of A. faecalis MOR02 and E. coli DH5α (carrying plasmid pT4-mCherry) on G. mellonella larvae.

Larvae treatment	Mortality (%)
A. faecalis MOR02-Cherry	24 h after injection	48 h after injection	72 h after injection	>96 h after injection
Puncture	0	0	0	0
LB broth	0	0	0	0
240 CFU	0	0	3.33 ± 4.71	0
2,400 CFU	0	6.67 ± 4.71^a	6.67 ± 9.43^a	33.33 ± 9.42^a
24,000 CFU	96.67 ± 4.71^a	3.33 ± 4.71^a	*	*
240,000 CFU	100^a	*	*	*
E. coli DH5α	24 h after injection	48 h after injection	72 h after injection	>96 h after injection
Puncture	0	10	0	0
LB broth	0	0	0	0
240 CFU	0	0	0	0
2,400 CFU	0	0	0	0
24,000 CFU	0	0	0	0
240,000 CFU	0	0	0	0

Significant differences between values. Note: the percentage values shown are accumulative. *At this time all larvae were dead.