Helicobacter pylori Outer Membrane Protein 18 (Hp1125) Is Involved in Persistent Colonization by Evading Interferon-γ Signaling

Table 1

Primers used in this study.

Primers used to construct Δomp18 strain
omp18-1: 5′-CCATCGATAACAAACGCTCTTTGGCTTC-3′	omp18-2: 5′-CGGAATTCGGCAATACCGATGAATTTGG-3′
omp18-3: 5′-CGGGATCCATGAAGAGATCTTCTGTATTTAG-3′	omp18-4: 5′-AAAACTGCAGTTACTTCATTAATTTGACATCC-3′
Primers for RT-PCR
napAF: 5′-TGAAGAGTTTGCGGACAT-3′	napAR: 5′-R AGAGTGGAAGCTCGTTTT-3′
cagAF: 5′-AGCAAAAAGCGACCTTGAA-3′	cagAR: 5′-AGCCAATTGCTCCTTTGAGA-3′
omp18F: 5′-TGCTTTTGGAAGGCAATACC-3′	omp18R: 5′-CATTTGGGTTTGGTTTCACC-3′
16SrRNAF: 5′-GCTCTTTACGCCCAGTGATTC-3′	16SrRNAR: 5′-GCGTGGAGGATGAAGGTTTT-3′

F: forward primer; R: reversed primer.
Underlined letters indicate nucleotides added at the 5′ end to create a restriction site.
Restriction sites for ClaI (omp18-1), EcoRI (omp18-2), BamHI (omp18-3), and PstI (omp18-4) are underlined letters.