Mcl-1 Is a Novel Target of miR-26b That Is Associated with the Apoptosis Induced by TRAIL in HCC Cells

Figure 1

HCC cell lines express high level of Mcl-1 and low level of miR-26b. Three independent experiments were performed. (a) The miR-26b expression levels of L02, HepG2, Hep3B, PLC, and Huh7 cells were detected by qPCR. versus LO2. (b) qPCR analysis for Mcl-1 mRNA expression level in all the above cell lines. versus LO2. (c) Western blot analysis for Mcl-1 protein expression level in all the above cell lines. (d) qPCR analysis for miR-26b levels in HCC cells after transfection with miR-26b mimics or NC oligonucleotide for 6 h. (e) qPCR analysis for Mcl-1 mRNA expression in HCC cells after transfection with miR-26b mimics or NC oligonucleotide for 24 h. . (f) Western blot analysis for Mcl-1 protein level in HCC cells after transfection with miR-26b mimics or NC oligonucleotide for 24 h.

(a)

(b)

(c)

(d)

(e)

(f)