Western blotting analysis of connexin 43 in heart in the presence of subanesthetic dose of sevoflurane, isoflurane, halothane, and propofol and no anesthetics (control) one hour after brain death and the sham group (without brain death). Representative Western blots showing phosphorylated connexin 43 and nonphosphorylated connexin 43 (a). The total connexin 43 calculated as the sum of phosphorylated and nonphosphorylated connexin 43. The total connexin level/β-actin level obtained in the sham group (without brain death) is normalized as 1.0 (b). The ratio of phosphorylated connexin 43/nonphosphorylated connexin 43 (c). The values are expressed as mean ± SD and the number of observations is 4 in each group.