Isolation, Culture, and Characterization of Chicken Cartilage Stem/Progenitor Cells

Figure 1

Cell morphology at different stages along with chicken CSPCs culture. (a) Chicken CSPCs on day 1 of primary culture, CSPCs cultured with some cartilage cells; (b) on day 3 of primary culture, the number of CSPCs increased and part of cartilage cells died; (c) CSPCs at P1. Cells increased quickly and cells aggregated in certain areas. (d) to (x) CSPCs at P2 to P22. During this time, cartilage cells died progressively and cells at P5 and P17 were homogeneous with a typical long spindle-shape. CSPCs at P18 appeared senescent.

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