Lysophosphatidic Acid Inhibits Apoptosis Induced by Cisplatin in Cervical Cancer Cells

Figure 3

Western blotting analysis of apoptotic proteins in cells treated with cisplatin. Hela cells were treated with cisplatin at different doses including 0.50 μg/mL, 100 μg/mL, 150 μg/mL, 200 μg/mL, and 250 μg/mL, for 4 hours. β-actin was used as a protein loading control. The resultant bands were subject to densitometry analysis for quantification. (a) Western blotting of Fas-L. The expression of Fas-L was significantly induced by cisplatin in a dose-dependent manner. (b) Western blotting of Bcl-2. The expression of Bcl-2 was significantly inhibited by cisplatin in a dose-dependent manner. (c) Western blotting of Bax. The expression of Bax was significantly induced by cisplatin in a dose-dependent manner.

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