Mammalian Cell Culture Process for Monoclonal Antibody Production: Nonlinear Modelling and Parameter Estimation

Table 1

Macroreactions of the mAb production process [4, 27].

Reaction number	Macroreaction scheme
1	GLC → 2LAC
2	GLC + 2GLU → 2ALA + 2LAC
3	GLC + 2GLU → 2ASP + 2LAC
4	GLU → PRO
5	ASN → ASP + NH3
6	GLN + ASP → ASN + GLU
7	0.0508GLC + 0.0577GLN + 0.0133ALA + 0.006ASN + 0.0201ASP + 0.0016GLU + 0.081PRO → BM
8	0.0104GLN + 0.011ALA + 0.072ASN + 0.082ASP + 0.0107GLU + 0.0148PRO → MAb
9	GLN → GLU + NH3