Research Article

Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice

Figure 9

HDL transport and cholesterol flux in endothelial cell culture studies. (a) Penetration of HDL labeled by C14-cholesteryl oleate through polarized human primary aortic EC, SVEC4-10, and EA.hy926 EC after 4 hrs of incubation. AP-HDL was placed into Transwell (apical side of the cells); BL-HDL was placed into basolateral compartment. The concentration of labeled HDL in media was 50 μg/mL (for protein). The specific activity of HDL was 2000 DPM/μg. Arrows show direction of HDL penetration through the cells: from apical to basolateral side (arrow pointing down) or in the opposite direction (arrow pointing up). Dark bars represent results for loading HDL into top compartment, whereas light grey bars show results for placing labeled HDL in the bottom compartment. Results are presented as the mean ± 1 SEM. ; . (b) Uptake of C14-cholesteryl ester from 14C-cholesteryl oleate-HDL by polarized human primary aortic EC, SVEC4-10, and EA.hy926 EC after 4 hours of incubation. Conditions of the experiment and bar labeling were the same as for (a). Results are presented as the mean ± 1 SEM. ; .
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