Mmu-miR-27a-5p-Dependent Upregulation of MCPIP1 Inhibits the Inflammatory Response in LPS-Induced RAW264.7 Macrophage Cells

Table 1

(a) Primers for MCPIP1 3′UTR, MCPIP1 3′-UTRm, and GAPDH 3′-UTR cloning. (b) Primers for MCPIP1 3′-UTR truncated mutation.

(a)

Number	Location	Sequence (5′-3′)
1	1–33	GGCGGAGCTCGCCAGAAGGTGGCGCAAGGGGCTGTCGGGCATC
2	796–766	GCGGTCGACCTGTAGCTGTCTTCAGACACCCCAGAAGAGG
3	1–19	GGCGGAGCTCGAAACCCTGGACCACCCAC
4	178–159	GCGGTCGACGGGTGCAGCGAACTTTATTG
5	451–511	TTCATGTTAGTAAGCAAGATGCTTCTTGTTTTTTCTCCCCTGCCCGCTCTGTCCACCTACA
6	511–451	TGTAGGTGGACAGAGCGGGCAGGGGAGAAAAAACAAGAAGCATCTTGCTTACTAACATGA

1: 3′-UTRMCPIP1-F; 2: 3′-UTRMCPIP1-R; 3: 3′-UTRGAPDH-F; 4: 3′-UTRGAPDH-R; 5: 3′-UTRMCPIP1-mF; 6: 3′-UTRMCPIP1-mR.

(b)

Number	Location	Sequence (5′-3′)
7	1–23	GCGCGAGCTCGCCAGAAGGTGGCGCAAGGGGCT
8	810–776	GCCGTCGACGTATGTAAGTACACTGTAGCTGTCTTCAGACACC
9	567–543	GCCGTCGACCACCCATGGGAGGTGTGGAAACAGA
10	410–387	GCCGTCGACGAGGGGGCCTCAGAGAGCAGGCAC
11	240–213	GCCGTCGACCTGACCGGCAGCGTCTCCTTAAATGAC

7: 3′-UTRMCPIP1-F2; 8: 3′-UTRMCPIP1-R810; 9: 3′-UTRMCPIP1-R567; 10: 3′-UTRMCPIP1-R410; 11: 3′-UTRMCPIP1-R230.