Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

Table 5

Inhibitory activity (U^a) of partial purified bacteriocin determined by the well-diffusion method against potential microbial pathogens associated with mastitis in dairy bovines.

Indicator bacteria	Bacteriocins
	Morricin 269	Kurstacin 287	Kenyacin 404	Entomocin 420	Tolworthcin 524
Bacillus cereus 183^b	151	365	264	264	264
Staphylococcus aureus	0	0	0	0	0
Streptococcus dysgalactiae	0	0	365	365	330
Staphylococcus agnetis	53	28	142	148	104
Staphylococcus epidermidis	0	0	0	0	0
Streptococcus uberis	204	296	264	296	233
Staphylococcus sciuri	0	0	0	0	0
Staphylococcus haemolyticus	0	0	0	0	0
Staphylococcus equorum	186	245	231	374	225
Brevibacterium stationis	62	28	329	204	150
Brachybacterium conglomeratum	103	44	296	480	150
Raoultella sp.	264	264	296	296	264

 ^aOne unit is defined as 1 mm² of the zone of inhibition as determined by the well-diffusion method (see text). Data are the average of triplicate assays. A value of “0” indicates no inhibition.
^bBacterium used as positive control. It was used to determine the units of bacteriocins contained in the crude extracts used in the assay [17, 18].