BioMed Research International / 2015 / Article / Fig 2

Research Article

Organotypic Culture of Breast Tumor Explants as a Multicellular System for the Screening of Natural Compounds with Antineoplastic Potential

Figure 2

Morphological integrity of tumor tissue explants cultured ex vivo. Histopathological findings of tumor tissue at zero time (basal) and cultivated during different times in DMEM/F12 supplemented medium show that neoplastic tissue remains viable. It is possible to observe cells in active mitosis (M), desmoplastic reaction (DR) adjacent to the tumor cells, and presence of inflammatory cells (arrow). Desmoplasia or desmoplastic reaction is usually only associated with malignant neoplasms, which can evoke a fibrosis response by invading healthy tissue. All these characteristics are typical of tumor tissue. (a) 0 h, (b) 24 h, (c) 48 h, (d) 72 h, and (e) 96 h in culture, respectively. H&E staining (40x).

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