BioMed Research International / 2015 / Article / Fig 3

Research Article

Organotypic Culture of Breast Tumor Explants as a Multicellular System for the Screening of Natural Compounds with Antineoplastic Potential

Figure 3

Nuclear expression of the cell proliferation marker Ki 67 in cultures of breast cancer explants. Representative images showing that over 40% of the neoplastic cells express Ki 67 at all-time points. The proliferation index is considered “high” when >20% of the cells are positive for this marker. (a) Normal breast tissue (negative control); (b) breast tumor (positive control); (c), (d), and (e) breast tumor explants cultured for 24, 48, and 72 h, respectively. Immunohistochemical staining (10x).
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