Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value

Figure 6

Real-time quantitative PCR validation. (a) Raw data of the expression levels of the four lncRNAs (LOC84740, ENST00000498296, AL359062, and ENST00000438550) based on microarray analysis. All four of these lncRNAs displayed a high basal expression level in metastatic and primary NPC tumors, but their expression levels significantly differed between the two groups. . (b) Validation of the microarray data. All four lncRNAs (LOC84740, ENST00000498296, AL359062, and ENST00000438550) were differentially expressed in the metastatic and primary NPC tumors based on microarray analysis, which was validated via QPCR using the same tissues. The validation results of the four lncRNAs indicated that the microarray data strongly correlated with the QPCR results. and . (c) The expression levels of four lncRNAs (LOC84740, ENST00000498296, AL359062, and ENST00000438550) were measured in an independent panel of 106 primary NPC samples via QPCR. These lncRNAs displayed higher expression levels in NPC primary tumors with progression than in those without progression. .

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