Loss of flfl Triggers JNK-Dependent Cell Death in Drosophila

Figure 5

Loss of flfl induces JNK pathway activation and cell death in wing development. Drosophila 3rd instar wing discs with AO ((a)–(c)) and X-Gal staining ((d)–(f)) are shown. Knocking down flfl in the posterior compartment of wing discs by en-Gal4 induced extensively cell death (c) and puc-LacZ expression (f), while expressing a GFP RNAi failed to do so ((b) and (d)). en-Gal4 ((a) and (d)) served as controls. Dashed line indicates the anterior-posterior boundary of wing discs ((c) and (f)). Anterior boundary is to the left in all panels. Genotypes: en-Gal4/+ (a); en-Gal4/UAS-GFP-IR (b); en-Gal4/UAS-flfl-IR (c); en-Gal4/+; /+ (d); en-Gal4/+; /UAS-GFP-IR (e); en-Gal4/+; /UAS-flfl-IR (f). SMEK1 (g) and DUSP1 (h) relative expression level in invasive breast carcinoma stroma compared to normal tissue in Finak Breast dataset are shown. Reporter: A_24_P36961 and A_23_P110712 are probes used in the study to detect SMEK1 and DUSP1, respectively. Breast stands for normal samples. The number in the parenthesis represents the total number of samples.