Vitamin A, Cancer Treatment and Prevention: The New Role of Cellular Retinol Binding Proteins

Figure 4

CRBP-1⁺ expression influences retinoid-induced A2780 cancer cell viability. (a) Viability of transfected CRBP-1 (CRBP-1⁺) A2780 cells increased after 4 days in the presence of 10% FBS compared to empty transfected (CRBP-1⁻) A2780 cells. (b) Retinol induces a reduction of viability in CRBP-1 A2780 cells after 24 h of different ATRA treatments (c) Flow cytometry analysis of Annexin V/PI apoptotic assay shows a higher percentage of dying cells in CRBP-1⁺ after 2 h of 20 µM retinol treatment compared to CRBP-1⁻ A2780 cells. Values are expressed as means ± SEM of three different experiments; , .

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