Molecular Detection of Theileria spp. in Livestock on Five Caribbean Islands

Table 2

Primers and probes used in this study.

PCR	Primer/probe	Nucleotides sequence	Amplicon
FRET-qPCR	Forward; Reverse; 6-FAM; LCRed-640	5′-TAGTGACAAGAAATAACAATACGGGGCTT-3′; 5′-CAGCAGAAATTCAACTACGAGCTTTTTAACT-3′; 5′-CCAATTGATACTCTGGAAGAGGTTT-(6-FAM)-3′; 5′-(LCRed640)-AATTCCCATCATTCCAATTACAAGAC-Phosphate-3′	178 bp
Conventional PCR	Upstream; Downstream	5′-CCTGAGAAACGGCTACCACATCT-3′; 5′-GGACTACGACGGTATCTGATCG-3′	T. orientalis 593 bp; T. buffeli 591 bp; T. annulata 591 bp; T. sergenti 591 bp; T. luwenshuni 594 bp; T. velifera 592 bp; T. ovis 595 bp; T. parva 592 bp; T. uilenbergi 592 bp; T. equi 596 bp; T. cervi 595 bp; T. lestoquardi 591 bp; T. separata 593 bp; T. capreoli 599 bp; T. bicornis 610 bp; T. taurotragi 587 bp; T. mutans 585 bp;  B. vulpes 609 bp; Theileria sp. OT3 600 bp; Theileria sp. NG 597 bp; Theileria sp. YW 593 bp; Theileria sp. B15 584 bp