Monitoring the Modifications of the Vitreous Humor Metabolite Profile after Death: An Animal Model

Figure 2

PCA applied to the ¹H NMR spectral data of VH samples withdrawn at different times after death (0 and 6, 12, 18, and 24 hours). (a) PC1 versus PC2 scores plot (in brackets, the explained variance). Green circles = samples at time 0; blue boxes = samples at 6 hours; red triangles = samples at 12 hours; yellow inverted triangles = samples at 18 hours; light blue diamonds = samples at 24 hours. Arrows are arbitrarily drawn and represent time-related trajectories. (b) PC1 versus PC2 loadings plot. Assignments of major contributions are given. 3-hydroxybutyrate (3-OH-But), lactate (Lac), alanine (Ala), acetate (Ace), glutathione in its reduced and oxidized forms (GSH/GSSG), creatine (Crt), choline/phosphocholine (Cho), glucose (Gluc), myo-inositol (MI), ascorbate (Asc), and hypoxanthine (Hpx).

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