Application of AMOR in Craniofacial Rabbit Bone Bioengineering

Figure 1

Micro-CT analysis of de novo bone formation within rabbit calvarial defects. Three-dimensional micro-CT images of bone specimens after 6 weeks of surgical creation of critical size defect (a). Representative sample of absorbable collagen sponge treated with plain PBS (Neg) or with immobilized antibodies. Antibodies included isotype matched control mAb (Iso), polyclonal anti-BMP-2 Ab (pAb), or BMP-2-specific mAb clones (C3, C15, C18, C20, C22, C24, and 3G7). Quantitative μCT of new bone volume (mm³) of respective controls and anti-BMP-2 clones are shown (b). Red highlighted area demarcates segmented volume corresponding to the area of the original defect. This highlighted area represents the volume which was measured during quantitative analysis. Results are presented as fold change and ± SD versus isotype antibody control ( for Neg, ISO, C22, 3G7 samples only). ^* or ^**.

(a)

(b)