Research Article

A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines

Figure 1

Cytotoxic effect of LfB and LfcinB25 in the OSCC tumorigenic cell lines CAL27 (a) and SCC15 (b) and the immortalized nontumorigenic keratinocytes cell line Het-1A (c). The cells were incubated for 24 h with the LfB protein and the LfcinB25 peptide. After treatment, cell viability was determined by MTT assay and calculated as the percentage of average absorbance of each treatment relative to the average absorbance of the negative control. The maximum concentration of the LfB protein was 1.25 μM (100 μg/mL) and of LfcinB25 32 μM (100 μg/mL). Each treatment was done in triplicate.
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