Research Article
A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines
Figure 3
Cytotoxic activity of LfcinB(20–25)4 in long-term treatments. (a) SCC15 and CAL27 cells were incubated with the peptide for 24, 48, 72, and 96 h. (b) SCC15 and CAL27 cells were treated for 24 h with the peptide and washed, and cells were incubated for 0, 24, 48, and 72 h in fresh culture medium. After the treatments, cell viability was determined by the MTT assay and calculated as the percentage of average absorbance of each treatment relative to the average absorbance of the negative control. Cell viability was evaluated 24, 48, and 72 h after treatments and was calculated as the percentage of average absorbance of treatments in relation to average absorbance of negative control. The concentrations of LfcinB(20–25)4 and STA used were 22.25 μM (100 μg/mL) and 0.86 μM (0.4 μg/mL), respectively. Treatments were done in triplicate.
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