Research Article
A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines
Figure 4
Cytotoxicity evaluation at different time points of treatment with LfcinB-derived peptides in the OSCC tumor cell lines CAL27 (a), SCC15 (b), and the immortalized nontumorigenic keratinocytes cell line Het-1A (c). The cells were incubated with LfcinB(20–25) or LfcinB(20–25)4 at the indicated times. After treatment, cell viability was determined by the MTT assay and calculated as the percentage of average absorbance of each treatment relative to the average absorbance of the negative control. The concentration of the LfcinB(20–25)4 used was 22.25 μM (100 μg/mL) and of LfcinB(20–25) was 101.5 μM (100 μg/mL). The STA concentration used was 0.86 μM (0.4 μg/mL) for CAL27 and 1.29 μM (0.6 μg/mL) for SCC15 and Het-1A. Each treatment was done in triplicate.
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