Research Article
A Tetrameric Peptide Derived from Bovine Lactoferricin Exhibits Specific Cytotoxic Effects against Oral Squamous-Cell Carcinoma Cell Lines
Figure 7
Assessment of necrosis/apoptosis in nontumorigenic cell line Het-1A (a) and the tumorigenic cell line CAL27 (b). Cells were detached and incubated with different peptides indicated for 1 h, after which they were labeled with both Annexin V-FITC and PI, and analyzed by flow cytometry. 10 μM (4.66 μg/mL) STA and 0.2% T-X100 were used as controls. The maximum concentration of the peptides used was 100 μg/mL equivalent to LfcinB25, 32 μM; LfcinB(20–25), 101.5 μM; LfcinB-Pal, 67.3 μM; and LfcinB(20–25)4, 22.25 μM. Photomicrographs were taken with a phase-contrast microscope. Barr = 100 μm.
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