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BioMed Research International
Volume 2015, Article ID 630461, 9 pages
Research Article

Behaviour of Endothelial Cells in a Tridimensional In Vitro Environment

1Centre de Recherche en Organogénèse Expérimentale de l’Université Laval/ LOEX, Québec, QC, Canada G1J 1Z4
2Division of Regenerative Medicine, CHU de Québec Research Centre, Quebec, QC, Canada G1J 1Z4
3Faculté de Pharmacie, Université Laval, Québec, QC, Canada G1V 0A6
4Département de Chimie, PROTEO, CERMA, Université Laval, Québec, QC, Canada G1V 0A6

Received 8 October 2014; Revised 25 January 2015; Accepted 25 January 2015

Academic Editor: Hannes Stockinger

Copyright © 2015 Raif Eren Ayata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Angiogenesis is a fundamental process in healing, tumor growth, and a variety of medical conditions. For this reason, in vitro angiogenesis is an area of interest for researchers. Additionally, in vitro angiogenesis is important for the survival of prevascularized tissue-engineering models. The aim of this study was to observe the self-tubular organization behaviour of endothelial cells in the self-assembly method. In this study, bilayered and dermal substitutes were prepared using the self-assembly method. Histological, immunostaining, and biochemical tests were performed. The behavioural dynamics of endothelial cells in this biological environment of supportive cells were observed, as were the steps of the in vitro angiogenic cascade with self-organizing capillary-like structures formation. The epidermal component of the substitutes was seen to promote network expansion and density. It also increased the quantity of angiogenic factors (VEGF and Ang-1) without increasing the proinflammatory factor (IL-8). In addition, the increased MMP activity contributed to matrix degradation, which facilitated capillary formation.