Role of TLR4-Mediated PI3K/AKT/GSK-3β Signaling Pathway in Apoptosis of Rat Hepatocytes

Figure 5

TLR4 inhibition, GSK-3β inhibition, and AKT inhibition affect GSK-3β translocation induced by LPS. The five groups were treated as above 4. GSK-3β translocated to the nucleus in LPS group (white arrow) and LY294002 + LPS group (yellow arrows). BRL-3A cells were incubated with GSK-3β antibody. Nucleus was visualized using Hoechst 33342 (blue). Immunofluorescence microscopy was used to detect the location of GSK-3β (green). Scale bars = 10 μm.