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BioMed Research International
Volume 2015, Article ID 635748, 8 pages
http://dx.doi.org/10.1155/2015/635748
Research Article

MicroRNA and mRNA Features of Malignant Pleural Mesothelioma and Benign Asbestos-Related Pleural Effusion

1Department of Chest Diseases, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
2Division of Thoracic Surgery, Department of Surgery, Mayo Clinic, Rochester, MN 55905, USA
3Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA
4Division of Oncology, National Jewish Health, Denver, CO 80206, USA
5Department of Public Health, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
6Department of Pathology, Medical Faculty, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey
7Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
8Division of Pulmonary & Critical Care Medicine, Mayo Clinic, Rochester, MN 55905, USA

Received 31 August 2014; Accepted 27 October 2014

Academic Editor: Seiichi Saito

Copyright © 2015 Guntulu Ak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant pleural mesothelioma or benign asbestos-related pleural effusion. Methods. Fresh frozen tissues from a total of 18 malignant pleural mesothelioma and 6 benign asbestos-related pleural effusion patients were studied. Expression profiling of mRNA and microRNA was performed using standard protocols. Results. We discovered significant upregulation of multiple microRNAs in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Hsa-miR-484, hsa-miR-320, hsa-let-7a, and hsa-miR-125a-5p were able to discriminate malignant from benign disease. Dynamically regulated mRNAs were also identified. MET was the most highly overexpressed gene in malignant pleural mesothelioma compared to benign asbestos-related pleural effusion. Integrated analyses examining microRNA-mRNA interactions suggested multiple altered targets within the Notch signaling pathway. Conclusions. Specific microRNAs and mRNAs may have diagnostic utility in differentiating patients with malignant pleural mesothelioma from benign asbestos-related pleural effusion. These studies may be particularly helpful in patients who reside in a region with a high incidence of mesothelioma.