Combination of Antiestrogens and Omega-3 Fatty Acids for Breast Cancer Prevention

Figure 1

(a) Carmine-stained abdominal-inguinal mammary gland whole mounts depicting the effect of increasing dietary n-3FA : n-6FA on breast histology, bars = 0.5 cm. (b) Mammary gland density analysis shows a decreasing trend in density as n-3FA : n-6FA increases. The methodology for measurement of mammary gland density is described in detail in our publication [18]. Briefly, whole mounts of the abdominal-inguinal mammary gland chains were photographed and the images obtained were digitized. Digital images of the whole mounts were captured using a semiautomated image acquisition system. Images were evaluated for total area of the mammary fat pad occupied by mammary epithelium as well as total area of the fat pad encompassed by the mammary ductal tree. Area occupied by the mammary epithelium divided by the total area encompassed by the mammary ductal tree was calculated. (c) Linear regression of mammary gland density and IGF-I upper prediction limit (UPL); upper confidence limit (UCL); estimate (EST); lower confidence limit (LCL); lower prediction limit (LPL). Reproduced with permission from [18].

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