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BioMed Research International
Volume 2015, Article ID 639521, 4 pages
http://dx.doi.org/10.1155/2015/639521
Research Article

Helicobacteraceae in Bulk Tank Milk of Dairy Herds from Northern Italy

1Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, 26900 Lodi, Italy
2Mouse and Animal Pathology Laboratory, Filarete Foundation, 20139 Milan, Italy
3Genomics Platform, Parco Tecnologico Padano, 26900 Lodi, Italy
4Department of Veterinary Science and Public Health, University of Milan, 20133 Milano, Italy

Received 29 September 2014; Revised 19 December 2014; Accepted 28 December 2014

Academic Editor: Khean-Lee Goh

Copyright © 2015 Valentina Bianchini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Helicobacter pylori is responsible for gastritis and gastric adenocarcinoma in humans, but the routes of transmission of this bacterium have not been clearly defined. Few studies led to supposing that H. pylori could be transmitted through raw milk, and no one investigated the presence of other Helicobacteraceae in milk. In the current work, the presence of Helicobacteraceae was investigated in the bulk tank milk of dairy cattle herds located in northern Italy both by direct plating onto H. pylori selective medium and by screening PCR for Helicobacteraceae, followed by specific PCRs for H. pylori, Wolinella spp., and “Candidatus Helicobacter bovis.” Three out of 163 bulk milk samples tested positive for Helicobacteraceae, but not for the subsequent PCRs. H. pylori was not isolated in any case. However, given similar growth conditions, Arcobacter butzleri, A. cryaerophilus, and A. skirrowii were recovered. In conclusion, the prevalence of Helicobacteraceae in raw milk was negligible (1.8%), and H. pylori was not identified in any of the positive samples, suggesting that, at least in the farming conditions of the investigated area, bovine milk does not represent a potential source of infection.