Clinical Safety and Immunogenicity of Tumor-Targeted, Plant-Made Id-KLH Conjugate Vaccines for Follicular Lymphoma
Table 5
Lack of immune reactivity of patient sera to plant glycans in idiotypic vaccines.
Patient UPIN1
Predominant plant glycan type2
Vaccinated patient sera
Control4 glycosylated antibody pre
Control4 glycosylated antibody post
Anti-HRP3
Anti-HRP3
Pre-immune
Post-immune
U001
V: GnGnXF S: Gn(FA)XF; (FA)(FA)XF
0.076
0.068
0.071
0.065
T003
V: GnGnXF S: Gn(FA)XF; (FA)(FA)XF
0.081
0.103
0.084
0.080
T006
V: GnGnXF S: Gn(FA)XF
0.106
0.109
0.072
0.068
T008
V: GnGnXF
0.107
0.174
0.100
0.089
T010
V: MGnX S: GnAF/Gn(FA)
0.089
0.105
0.079
0.074
U011
V: GnGnXF S: Gn(FA)XF
0.078
0.069
0.156
0.130
U016
V: GnGnXF S: Gn(FA)XF
0.096
0.082
0.066
0.057
U017G
V: GnGnXF S: Gn(FA)XF
0.094
0.078
0.101
0.064
T021
V: GnGnXF
0.142
0.103
0.072
0.078
T022
V: GnGnXF
0.089
0.136
0.085
0.083
U026
V: GnGnXF S: GnA
0.067
0.069
0.062
0.066
Control anti-plant glycan antiserum (1 : 2000)
0.37
0.515
1 = UPIN: Unique Patient Identification Number (sera obtained from patients vaccinated as shown in Table 4). 2 = Predominant plant glycan type. V, vacuolar, with major species indicated; S, secretory, with major species indicated. A = galactose; Gn = N-acetyl glucosamine; M = mannose; F = fucose; X = xylose. 3 = HRP, horseradish peroxidase, a plant enzyme containing complex plant-type glycans used as a control. 4 = Irrelevant plant-produced glycosylated human idiotypic Ig used as a control.
