Triphala Extract Suppresses Proliferation and Induces Apoptosis in Human Colon Cancer Stem Cells via Suppressing c-Myc/Cyclin D1 and Elevation of Bax/Bcl-2 Ratio

Figure 3

Antiproliferative efficacy of methanol extract of triphala (MET) on HCCSCs with functioning p53 and shRNA-attenuated p53 (a) and p53^+/+ and p53^−/− cells of HCT116 (b). Cells were treated with either 5-fluorouracil (FU, 25, 50, or 100 μg/mL) or MET (25, 50, 100, or 200 μg/mL) for 20 hours and BrdU assay was performed as described in methods. Results were expressed as mean ± SE for three experiments at each time point. The error bars with the same letter indicate no significance at .

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(b)