Figure 3: Antiproliferative efficacy of methanol extract of triphala (MET) on HCCSCs with functioning p53 and shRNA-attenuated p53 (a) and p53+/+ and p53−/− cells of HCT116 (b). Cells were treated with either 5-fluorouracil (FU, 25, 50, or 100 μg/mL) or MET (25, 50, 100, or 200 μg/mL) for 20 hours and BrdU assay was performed as described in methods. Results were expressed as mean ± SE for three experiments at each time point. The error bars with the same letter indicate no significance at .