Figure 5: Effect of methanol extract of triphala (MET) on pro- and antiapoptotic protein, Bax, and Bcl-2 expression. HCCSCs were treated with MET (104 μg/mL) and 5-fluorouracil (75 μg/mL). Cytoplasmic proteins were extracted by using NE-PER cytoplasmic extraction kit (Pierce) and then analyzed using western blotting for proapoptotic protein, Bax, and antiapoptotic protein, Bcl-2. Data are representative of three individual experiments; a representative blot is presented. M: media control, S: solvent control, FU: 5-fluorouracil, and MET: methanol extract of triphala. The error bars with the same letter indicate no significance at .