Figure 7: Effect of methanol extract of triphala (MET) on c-Myc/cyclin D1 in HCCSCs. HCCSCs were treated for 24 hours with MET (104 μg/mL) and 5-fluorouracil (FU, 75 μg/mL). Nuclear proteins were extracted by using NE-PER cytoplasmic extraction kit and then analyzed by western blot for c-Myc, cyclin D1, and DNA topoisomerase IIβ. Data are representative of three individual experiments. M: media control, S: solvent control, FU: 5-fluorouracil, and MET: ethanol extract of triphala. The error bars with the same letter indicate no significance at .