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BioMed Research International
Volume 2015, Article ID 651464, 7 pages
Research Article

Studies on Molecular Characterizations of the Outer Membrane Proteins, Lipids Profile, and Exopolysaccharides of Antibiotic Resistant Strain Pseudomonas aeruginosa

1Food Science and Nutrition Department, College of Food and Agriculture Sciences, King Saud University, Saudi Arabia
2Food Science and Nutrition, Faculty of Home Economics, Helwan University, Egypt
3Department of Botany (Microbiology), Faculty of Science, Zagazig University, Zagazig, Egypt

Received 29 September 2014; Accepted 24 November 2014

Academic Editor: Anjali Joshi

Copyright © 2015 Hany M. Yehia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. M. D. Baron, Medical Microbiology, Library of Congress Cataloging-in-Publication Data Medical Microbiology, The University of Texas Medical Branch at Galveston, Galveston, Tex, USA, 4th edition, 1996.
  2. W. Levinson and E. Jawetz, “Gram negative rods related to enteric tract,” in Medical Microbiology and Immunology (Examination and Board Review), pp. 107–126, Lange Medical Books/McGraw-Hill, Toronto, Canada, 6th edition, 2000. View at Google Scholar
  3. N. S. Egorov, Antibiotics a Scientific Approach, MIR Publishers, Moscow, Russia, 1985.
  4. R. E. W. Hancock and A. Bell, “Antibiotic uptake into gram-negative bacteria,” European Journal of Clinical Microbiology & Infectious Diseases, vol. 7, no. 6, pp. 713–720, 1988. View at Publisher · View at Google Scholar · View at Scopus
  5. T. E. Cloete, “Resistance mechanisms of bacteria to antimicrobial compounds,” International Biodeterioration and Biodegradation, vol. 51, no. 4, pp. 277–282, 2003. View at Publisher · View at Google Scholar · View at Scopus
  6. R. Margesin and F. Schinner, “Decontamination of oil spills in cold environment,” Journal of Chemical Technology and Biotechnology, vol. 74, pp. 1–9, 1999. View at Google Scholar
  7. C. de Castro, R. Lanzetta, A. Molinaro, M. Parrilli, and V. Piscopo, “Acetyl substitution of the O-specific polysaccharide caryophyllan from the phenol phase of Pseudomonas (Burkholderia) caryophylli,” Carbohydrate Research, vol. 335, no. 3, pp. 205–211, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. M. Shigeta, G. Tanaka, H. Komatsuzawa, M. Sugai, H. Suginaka, and T. Usui, “Permeation of antimicrobial agents through Pseudomonas aeruginosa biofilms: a simple method,” Chemotherapy, vol. 43, no. 5, pp. 340–345, 1997. View at Publisher · View at Google Scholar · View at Scopus
  9. S. K. Kazy, P. Sar, S. P. Singh, A. K. Sen, and S. F. D'Souza, “Extracellular polysaccharides of a copper-sensitive and a copper-resistant Pseudomonas aeruginosa strain: synthesis, chemical nature and copper binding,” World Journal of Microbiology and Biotechnology, vol. 18, no. 6, pp. 583–588, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Bonilla, C. Olivaro, M. Corona, and M. A. Vazquez, “Soubes production and characterization of new bioemulsifier form Pseudomonas putida ML2,” Journal of Applied Microbiology, vol. 98, pp. 456–463, 2005. View at Google Scholar
  11. H. Eagle and A. Musselman, “The rate of bactericidal action of penicillin in vitro as a function of its concentration and its paradoxically reduced activity at high concentrations against certain organisms,” The Journal of Experimental Medicine, vol. 88, no. 1, article 99, 1948. View at Publisher · View at Google Scholar
  12. O. H. Lowry, N. J. Rosenbrough, A. L. Farr, and R. J. Randali, “Protein measurement with the Folin-phenol reagent,” The Journal of Biological Chemistry, vol. 193, no. 1, pp. 265–275, 1951. View at Google Scholar · View at Scopus
  13. H. G. Deneer and A. A. Potter, “Iron-repressible outer-membrane proteins of Pasteurella haemolytica,” Journal of General Microbiology, vol. 135, pp. 435–443, 1989. View at Google Scholar · View at Scopus
  14. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  15. E. G. Bligh and W. J. Dyer, “A rapid method of total lipid extraction and purification,” Canadian Journal of Biochemistry and Physiology, vol. 37, no. 8, pp. 911–917, 1959. View at Publisher · View at Google Scholar · View at Scopus
  16. M. Kates, “Techniques of lipodology,” in Laboratory Techniques in Biochemistry and Molecular Biology, T. S. Work and E. Work, Eds., p. 341, North-Holland, London, UK, 1972. View at Google Scholar
  17. J. D. Weete, A. El Moughith, and J.-M. Touze-Soulet, “Inhibition of growth, lipid, and sterol biosynthesis by monensin in fungi,” Experimental Mycology, vol. 13, no. 1, pp. 85–94, 1989. View at Publisher · View at Google Scholar · View at Scopus
  18. K. P. Hebber, B. Gueniot, A. Heyroud et al., “Characetrization of exo-polysaccharides produced by rhizobacteria,” Applied Microbiology and Biotechnology, vol. 37, pp. 981–986, 1992. View at Google Scholar
  19. D. B. Duncan, “Multiple range and multiple F tests,” Biometrics, vol. 11, pp. 1–42, 1955. View at Google Scholar · View at MathSciNet
  20. C. Dytham, Choosing and Statistics: A Biologist's Guide, Blackwell Sceince, London, UK, 1999.
  21. A. Nakajima, Y. Sugimoto, H. Yoneyama, and T. Nakae, “High-level fluoroquinolone resistance in Pseudomonas aeruginosa due to interplay of the MexAB-OprM efflux pump and the DNA gyrase mutation,” Microbiology and Immunology, vol. 46, no. 6, pp. 391–395, 2002. View at Publisher · View at Google Scholar · View at Scopus
  22. J. A. Lomholt and M. Kilian, “Ciprofloxacin susceptibility of Pseudomonas aeruginosa isolates from keratitis,” British Journal of Ophthalmology, vol. 87, no. 10, pp. 1238–1240, 2003. View at Publisher · View at Google Scholar · View at Scopus
  23. M. C. Roberts, “Update on acquired tetracycline resistance genes,” FEMS Microbiology Letters, vol. 245, no. 2, pp. 195–203, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. W. A. Hassanein, Studies on the biological changes inducing by certain antibiotics and Gamma radiation for certain bacteria [Ph.D. thesis of Science in Microbiology], Faculty of Science, Zagazig University, 1994.
  25. L. Vaczi, The Biological Role of Bacterial Lipids, Akademiai Kiado, Budapest, Hungary, 1973.
  26. E. Drenkard, “Antimicrobial resistance of Pseudomonas aeruginosa biofilms,” Microbes and Infection, vol. 5, no. 13, pp. 1213–1219, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. J. D. Vrany, P. S. Stewart, and P. A. Suci, “Comparison of recalcitrance to ciprofloxacin and levofloxacin exhibited by Pseudomonas aeruginosa bofilms displaying rapid-transport characteristics,” Antimicrobial Agents and Chemotherapy, vol. 41, no. 6, pp. 1352–1358, 1997. View at Google Scholar · View at Scopus
  28. B. D. Hoyle and J. W. Costerton, “Bacterial resistance to antibiotics: the role of biofilms,” Progress in Drug Research, vol. 37, pp. 91–105, 1991. View at Google Scholar · View at Scopus