Ring-Oxidative Biotransformation and Drug Interactions of Propofol in the Livers of Rats

Figure 3

Ring-oxidative metabolism of propofol (PPF) into 4-hydroxypropofol. After reacting propofol with control or phenobarbital-treated liver microsomes in an NADPH-generation system, the metabolites were extracted with cyclohexane and dried with nitrogen gas. The extracted metabolites were dissolved in a mobile phase for the HPLC analyses (a). The peaks observed in the metabolism profile were quantified and analyzed (b). Time-dependent effects of propofol metabolism were determined (c). Each value represents the mean ± SD for . “^∗” Values significantly differ from the respective control, .

(a)

(b)

(c)