Ring-Oxidative Biotransformation and Drug Interactions of Propofol in the Livers of Rats

Figure 4

Roles of CYP2B1/2 in propofol hydroxylation verified by a chemical inhibition assay. Orphenadrine, an inhibitor of CYP2B1/2, was incubated with pentoxyresorufin or propofol. PROD activity was assayed by measuring the fluorescent product, resorufin (a). After reacting orphenadrine with propofol in phenobarbital (PB)-treated liver microsomes, the extracted metabolites were extracted, dried, and dissolved in the mobile phase for HPLC analyses (b). The concentration-dependent effects of orphenadrine on the metabolism of propofol into 4-hydroxypropofol were determined (c). Each value represents the mean ± SD for . “^∗” Values significantly differ from the respective control, .

(a)

(b)

(c)