Research Article
Ring-Oxidative Biotransformation and Drug Interactions of Propofol in the Livers of Rats
Figure 5
Roles of CYP2B1/2 in propofol hydroxylation verified by an immunoinhibition assay. A polyclonal antibody against CYP2B1/2 at 0.1, 0.2, and 0.3 mg was incubated with pentoxyresorufin or propofol. Pentoxyresorufin O-dealkylase (PROD) activity was assayed by measuring the fluorescent product, resorufin (a). After reacting 0.1, 0.2, and 0.3 mg of the CYP2B1/2 antibody with propofol in phenobarbital-treated liver microsomes, the extracted metabolites were extracted, dried, and dissolved in a mobile phase for the HPLC analyses. The HPLC results were quantified and statistically analyzed (b). Each value represents the mean ± SD for . “∗” Values significantly differ from the respective control, .
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