Endothelial Progenitor Cell Fraction Contained in Bone Marrow-Derived Mesenchymal Stem Cell Populations Impairs Osteogenic Differentiation

Table 1

Genes of interest were detected by Polymerase Chain Reaction (PCR) using specific oligonucleotide primers, TaqMan probes, or Assays-on-Demand. Eukaryotic 18S was used as a housekeeping gene.

Microsynth (target gene sequence (5′3′))	Applied Biosystems
Bone marker genes	Bone marker genes; ALP: Hs00758162_m1; Endothelial marker genes; PECAM-I: HS01065282_m1; vWF: Hs00169795_m1; TEK: Hs00176096_m1; Flt1: Hs00176573_m1; KDR: Hs00176676_m1; HOX9: Hs00365956_m1; Housekeeping gene; 18s: 4319413E
Collagen I1; Forw CCC TGG AAA GAA TGG AGA TGA T; Rev ACT GAA ACC TCT GTG TCC CTT CA; Probe CGG GCA ATC CTC GAG CAC CCT
Osteonectin
Forw ATC TTC CCT GTA CAC TGG CAG TTC; Rev CTC GGT GTG GGA GAG GTA CC; Probe CAG CTG GAC CAG CAC CCC ATT GAC
BMP-2
Forw AAC ACT GTG CGC AGC TTC C; Rev CTC CGG GTT GTT TTC CCA C; Probe CCA TGA AGA ATC TTT GGA AGA ACT ACC AGA AAC TG
Osteopontin
Forw CTC AGG CCA GTT GCA GCC; Rev CAA AAG CAA ATC ACT GCA ATT CTC; Probe AAA CGC CGA CCA AGG AAA ACT CAC TAC C
Runx2
Forw AGC AAG GTT CAA CGA TCT GAG AT; Rev TTT GTG AAG ACG GTT ATG GTC AA; Probe TGA AAC TCT TGC CTC GTC CAC TCC G
BSP II
Forw TGC CTT GAG CCT GCT TCC; Rev GCA AAA TTA AAG CAG TCT TCA TTT TG; Probe CTC CAG GAC TGC CAG AGG AAG CAA TCA