Phenotypic and Functional Properties of Porcine Dedifferentiated Fat Cells during the Long-Term Culture In Vitro

Figure 1

Morphological and gene expression changes during the dedifferentiation from porcine mature adipocytes to DFAT cells by ceiling culture. (a) Morphological changes of the dedifferentiation process. During the ceiling culture, mature adipocytes contained large single lipid droplets attached to the flasks firmly (A) at days 1-2 and the cells became oval shape at day 3 (B). Then, the lipid droplets were released and extruded out the cells at days 6–10 (C–E). Finally, the cells changed into fibroblast-like DFAT cells (F), bar, 50 μm. (b) Primary DFAT cells at P1 after trypsinization, bar, 200 μm. (c) The gene expression of adipogenic markers between mature adipocytes (MAs) and DFAT cells. The mRNA relative levels of qPCR PPARγ, aP2, LPL, and Adiponectin were detected by qPCR. Values are the mean ± SD of triplicate dishes.  .

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