A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

Table 2

Determination of MIA cut-off, diagnostic sensitivity, and diagnostic specificity for WNV.sE, WNV.EDIII, JEV.EDIII, and TBEV.EDIII antigens by ROC analysis on ELISA positive and negative samples.

	ROC analysis
	Number of ELISA positive samples	Number of ELISA negative samples	ROC cut-off	Diagnostic sensitivity determined with this cut-off	Diagnostic specificity determined with this cut-off
WNV.sE	189	172	17	98.4	99.4
WNV.EDIII	101	172	54	97.0	92.3
JEV.EDIII	88	172	55	93.2	94.7
TBEV.EDIII	59	172	61	98.4	100.0