BioMed Research International / 2015 / Article / Tab 4

Research Article

A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

Table 4

Comparison of the identification of circulating flaviviruses in equine field sera by three different techniques: flavivirus ELISA, MIA, and VNT.

Positive
sera
ELISAMIAFlavivirus
identification
VNT
Number of field seraELISA
pos.
ELISA
doubt.
ELISA
neg.
WNV.sE
pos.
WNV.EDIII
pos.
JEV.EDIII
pos.
TBEV.EDIII
pos.
Neg.WNV
pos.
JEV
pos
USUV
pos.
TBEV
pos.
Neg.Flavivirus
identification

WNV103: Pak. (68) + Mada. (35)1012 010099532396WNV positive99369/35
(Mada.)
NA496WNV positive
4Undetermined
flavivirus
3Undetermined
flavivirus
JEV101 (Japan)882 1190298721182JEV positive1196NANA591JEV positive
3WNV positive 1WNV positive
5Undetermined flavivirus4Undetermined Flavivirus
TBEV74 (Austria)593 125917611360TBEV positive3NA4621262TBEV positive
1Undetermined
flavivirus

Negative
sera
160 (Camargue + Ireland)160216120158158Negative
2WNV positive

Not analysed (NA).
Positive sera evaluated as false reactives for JEV or WNV if WNV.sE signal was negative.
Bold italic: negative sample and italic: doubtful sample.
The thresholds for ELISA, MIA, and MNT were as defined in Table 3. In the event of cross-reaction with rEDIII beads during MIA, a horse was considered infected with a specific flavivirus if the corresponding bead coupled to rEDIII generated an S/P ratio at least 1.5-fold greater than that generated with the other rEDIII beads. In the event of cross-reaction with MNT, flavivirus identification is determined by the virus with the highest neutralisation capacity and at least a fourfold difference in titres.