Inhibition of Osteoclast Activation by Phloretin through Disturbing αvβ3 Integrin-c-Src Pathway

Figure 6

Inhibition of the induction of paxillin and c-Cbl in ovariectomized (OVX) mouse femoral bone tissues (a and b). OVX mice were orally treated with 10 mg/kg/day phloretin daily for 8 weeks. Immunohistochemical staining was conducted by using a primary antibody of mouse paxillin (brown spot staining) and by counterstaining with methyl green. Representative images were visualized under light microscopy (3 separate experiments). Magnification: 40-fold. Femoral bone tissue extracts were subject to SDS-PAGE and Western blot analysis with a primary antibody against mouse c-Cbl. Representative blot data were obtained from three independent experiments, and β-actin protein was used as an internal control. The bar graphs (mean ± SEM) in the right panels represent quantitative results of blots obtained from a densitometer. Respective values not sharing a common letter are different at .

(a)

(b)