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BioMed Research International
Volume 2015 (2015), Article ID 680615, 17 pages
http://dx.doi.org/10.1155/2015/680615
Research Article

Improvement of Endurance of DMD Animal Model Using Natural Polyphenols

1Laboratorio Cellule Staminali, Dipartimento di Patofisiologia e dei Trapianti, Universitá degli Studi di Milano, Fondazione IRCCS Cá Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Via Francesco Sforza 35, 20122 Milano, Italy
2Sezione di Neuroscienze, Dipartimento di Patofisiologia e dei Trapianti, Universitá degli Studi di Milano, Fondazione IRCCS Cà Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Via Francesco Sforza 35, 20122 Milano, Italy
3Ystem S.r.l., Milano, Italy
4U.G.A. Nutraceuticals, Gubbio, Perugia, Italy

Received 30 May 2014; Revised 13 August 2014; Accepted 27 August 2014

Academic Editor: Giuseppe D’Antona

Copyright © 2015 Clementina Sitzia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Duchenne muscular dystrophy (DMD), the most common form of muscular dystrophy, is characterized by muscular wasting caused by dystrophin deficiency that ultimately ends in force reduction and premature death. In addition to primary genetic defect, several mechanisms contribute to DMD pathogenesis. Recently, antioxidant supplementation was shown to be effective in the treatment of multiple diseases including muscular dystrophy. Different mechanisms were hypothesized such as reduced hydroxyl radicals, nuclear factor-κB deactivation, and NO protection from inactivation. Following these promising evidences, we investigated the effect of the administration of a mix of dietary natural polyphenols (ProAbe) on dystrophic mdx mice in terms of muscular architecture and functionality. We observed a reduction of muscle fibrosis deposition and myofiber necrosis together with an amelioration of vascularization. More importantly, the recovery of the morphological features of dystrophic muscle leads to an improvement of the endurance of treated dystrophic mice. Our data confirmed that ProAbe-based diet may represent a strategy to coadjuvate the treatment of DMD.