Improvement of Endurance of DMD Animal Model Using Natural Polyphenols

Figure 6

Stem cell mobilization driven by ProAbe. (a) Histograms representing FACS analysis of percentage of stem cell and adhesion marker in PBMCs from treated C57Bl and mdx mice. T0 (before treatment) was used as baseline; bars represented the fold increase of cell’s percentage relative to T0. At T24 h, both mdx and C57Bl mice showed an increase in the percentage of peripheral CD34+, Sca-1+, and CXCR4+ cells. After 72 h of treatment the percentage of peripheral CD34+ and CXCR4+ started to decline. After 10 days of treatment the percentage of markers analyzed was similar to T0 levels. (b) FACS analysis, showing the percentage of SDF-1+ cells in QA, was higher in both the mice fed with ProAbe; in particular, in dystrophic mice it was more than 3-fold higher. (c) FACS analysis showing that the percentage of CD34+Sca-1+CD45+ cells isolated from QA was significantly higher only in the mdx-treated mice. (d) FACS analysis showing the percentage of CD34+Sca-1+CD45− cells isolated from treated and untreated mdx mice.

(a)

(b)

(c)

(d)