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BioMed Research International
Volume 2015, Article ID 681456, 9 pages
http://dx.doi.org/10.1155/2015/681456
Research Article

Osteoarticular Expression of Musashi-1 in an Experimental Model of Arthritis

1Department of Pathology and IBIMER, School of Medicine, University of Granada, 18012 Granada, Spain
2The Spanish Institute of Social Security (INSS), 18006 Granada, Spain
3Oral Surgery and Implant Dentistry Department, School of Dentistry, University of Granada, 18017 Granada, Spain
4Plastic Surgery Department, Virgen de las Nieves University Hospital, 18014 Granada, Spain
5Periodontics Department, School of Dentistry, University of Granada, 18017 Granada, Spain
6Parasitology and Biomedicine López-Neyra Institute, CSIC, 18016 Armilla, Granada, Spain
7Orthopedic Surgery Department, San Cecilio University Hospital of Granada, 18012 Granada, Spain

Received 23 September 2014; Revised 11 January 2015; Accepted 20 January 2015

Academic Editor: Monica Fedele

Copyright © 2015 Francisco O’Valle et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Collagen-induced arthritis (CIA), a murine experimental disease model induced by immunization with type II collagen (CII), is used to evaluate novel therapeutic strategies for rheumatoid arthritis. Adult stem cell marker Musashi-1 (Msi1) plays an important role in regulating the maintenance and differentiation of stem/precursor cells. The objectives of this investigation were to perform a morphological study of the experimental CIA model, evaluate the effect of TNFα-blocker (etanercept) treatment, and determine the immunohistochemical expression of Msi1 protein. Methods. CIA was induced in 50 male DBA1/J mice for analyses of tissue and serum cytokine; clinical and morphological lesions in limbs; and immunohistochemical expression of Msi1. Results. Clinically, TNFα-blocker treatment attenuated CIA on day 32 after immunization (). Msi1 protein expression was significantly higher in joints damaged by CIA than in those with no lesions () and was related to the severity of the lesions (Spearman’s rho = 0.775, ). Conclusions. Treatment with etanercept attenuates osteoarticular lesions in the murine CIA model. Osteoarticular expression of Msi1 protein is increased in joints with CIA-induced lesion and absent in nonlesioned joints, suggesting that this protein is expressed when the lesion is produced in order to favor tissue repair.