Construction of a gE-Deleted Pseudorabies Virus and Its Efficacy to the New-Emerging Variant PRV Challenge in the Form of Killed Vaccine

Table 1

Sequences of oligonucleotides used in PCR. Underlined nucleotide sequences are corresponding restriction enzyme sites.

Primer	Sequence	Details
TKA-F	5′-CCGgaattcCAAACACCAGCAGGGGCACGA-3′	Left homologous arm of TK gene
TKA-R	5′-CTAGtctagaATAACTTCGTATAATGTATGCTATACGAAGTTATTGTTGACCAGCATGGCGTAGACG-3′
TKB-F	5′-ACATgcatgcAACGACGACGGCGTGGGAGG-3′	Right homologous arm of TK gene
TKB-R	5′-CCCaagcttAGGGCGACGGCGAAGAAGAGC-3′
CMVU	5′-ACGCGTCGACTAGTTATTAATAGTAATCAATTACG-3′
SV40R	5′-GGCCGACGTCGACCTAGAATGCAGTGAAAAAAATGC-3′
gpt-F	5′-CCGCTCGAGCTATGAGCGAAAAATACATCGTCAC-3′
gpt-R	5′-CGCGGATCCGCGACCGGAGATTGGCGGGACGA-3′
PRVgE-F	5′-CTTCCACTCGCAGCTCTTCTC-3′	PCR to detect virus shedding from nasal swabs
PRVgE-R	5′-GTTAAGTTCTCGCGCGAGT-3′