Evaluation of Transduction Properties of an Adenovirus Vector in Neonatal Mice

Figure 2

Transduction property of an Ad vector in neonatal mice. (a) Luciferase expression levels in the organs following systemic administration of Ad-L2. Neonatal and adult mice were administered Ad-L2 at a dose of 1.2 × 10¹¹ IFU/kg (low dose) or 5.9 × 10¹¹ IFU/kg (high dose) via retro-orbital sinus. Luciferase production was examined in each organ 48 h after Ad vector administration. (b) Liver-specific luciferase production in neonatal mice following systemic administration of Ad-AHAL2. Neonatal mice received Ad vectors at a dose of 5.9 × 10¹¹ IFU/kg. Luciferase activities in the organs were measured as described in (a). (c) Ad vector genome copy numbers in the organs following systemic administration in the neonatal and adult mice. Mice were administered Ad-L2 at a dose of 5.9 × 10¹¹ IFU/kg via retro-orbital sinus. Ad genome copy numbers in each organ were examined 48 h after injection. The PBS-administered group had <10⁴ Ad genome copies/μg tissue DNA. (d, e) Duration of transgene expression following systemic administration of Ad-AHAL2 in neonatal mice. In vivo bioluminescence images are shown in (d). Bioluminescence intensity levels of the mice are graphically illustrated in (e). Neonatal mice were administered Ad-AHAL2 at a dose of 5.9 × 10¹¹ IFU/kg via retro-orbital sinus. Pseudocolor scales were represented as counts unit. Note that the legend of the pseudocolor scale on day 5 is different from that on the other days (N.D., not detected) ( animals per group for all experiments).

(a)

(b)

(c)

(d)

(e)