Generation and Characterization of a Transgenic Mouse Carrying a Functional Human β-Globin Gene with the IVSI-6 Thalassemia Mutation

Table 1

PCR primers employed for identification and characterization of transgenic mice.

Name	Sequence	Length (nt)	Melting temperature (°C)	Gene
MuActF	5′ TGACGGGGTCAACCACACTGTGCCCATCTA 3′	30	81	Murine β-actin
MuActR	5′ CTAGAAGCATTTGCGGTGGACGATGGAGGG 3′	30	80	Murine β-actin
TransF	5′ TGCATTCATTTGTTGTTGTTTTTCT 3′	25	65	Transgene (LCR)
TransF [6FAM]	5′ [6-FAM] TGCATTCATTTGTTGTTGTTTTTCT 3′	25	65	Transgene (LCR)
TransR	5′ TGACTAAAACTCCACCTCAAACGG 3′	24	67	Transgene (LCR)
HuBetaF	5′ AGACCTCACCCTGTGGAGCC 3′	20	68	Human β-globin
HuBetaR	5′ TCAGGAGTGGACAGATCCCC 3′	20	67	Human β-globin
MuActF1 [6FAM]	5′ [6-FAM] TACTTTGGGAGTGGCAAGCC 3′	20	66	Murine β-actin
MuActR1	5′ TCTCCATGTCGTCCCAGTTG 3′	20	66	Murine β-actin