Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs
Table 3
Experimental design and results of the Plackett-Burman design.
Trials
Variable levels
Yield (mg/L)
Observed
Predicted
1
1
−1
1
1
−1
−1
−1
−1
1
−1
88.38 ± 3.84
90.91
2
1
1
−1
1
1
−1
−1
−1
−1
1
117.96 ± 2.37
115.41
3
−1
1
1
−1
1
1
−1
−1
−1
−1
97.42 ± 6.78
96.55
4
−1
−1
1
1
−1
1
1
−1
−1
−1
101.79 ± 3.41
98.33
5
1
−1
−1
1
1
−1
1
1
−1
−1
102.43 ± 3.74
99.73
6
1
1
−1
−1
1
1
−1
1
1
−1
92.45 ± 3.96
94.17
7
1
1
1
−1
−1
1
1
−1
1
1
107.47 ± 9.89
104.83
8
1
1
1
1
−1
−1
1
1
−1
1
121.38 ± 2.26
124.57
9
−1
1
1
1
1
−1
−1
1
1
−1
119.62 ± 2.27
117.05
10
1
−1
1
1
1
1
−1
−1
1
1
88.76 ± 5.85
90.81
11
−1
1
−1
1
1
1
1
−1
−1
1
119.63 ± 5.56
123.21
12
1
−1
1
−1
1
1
1
1
−1
−1
79.81 ± 4.77
80.87
13
−1
1
−1
1
−1
1
1
1
1
−1
118.85 ± 5.84
120.81
14
−1
−1
1
−1
1
−1
1
1
1
1
76.33 ± 3.15
77.53
15
−1
−1
−1
1
−1
1
−1
1
1
1
82.21 ± 2.79
80.17
16
−1
−1
−1
−1
1
−1
1
−1
1
1
77.19 ± 4.41
76.27
17
1
−1
−1
−1
−1
1
−1
1
−1
1
62.37 ± 5.76
61.05
18
1
1
−1
−1
−1
−1
1
−1
1
−1
106.22 ± 7.25
104.95
19
−1
1
1
−1
−1
−1
−1
1
−1
1
90.56 ± 2.95
90.05
20
−1
−1
−1
−1
−1
−1
−1
−1
−1
−1
60.31 ± 3.20
63.93
Each experiment was repeated three times, and all of the data were expressed as means ± standard deviations. X1, X2, X3, X4, X5, X6, X7, X8, X9, and X10 represent peptone (g/L), corn powder (g/L), starch (g/L), soybean meal (g/L), KH2PO4 (g/L), (NH4)2SO4 (g/L), incubation temperature (°C), initial pH, incubation time (h), and inoculum size (%).
