Improved Production of Sublancin 168 Biosynthesized by Bacillus subtilis 168 Using Chemometric Methodology and Statistical Experimental Designs

Table 5

Experimental design and results of Box-Behnken optimization experiment.

Trials				Yield (mg/L)
				Observed	Predicted
1	22.00	24.00	36.00	73.66 ± 1.62	73.43
2	34.00	28.00	32.00	86.35 ± 2.78	86.78
3	28.00	28.00	28.00	77.37 ± 1.83	77.57
4	28.00	24.00	32.00	124.39 ± 1.92	124.15
5	22.00	28.00	32.00	71.97 ± 1.26	71.81
6	34.00	24.00	28.00	94.52 ± 3.73	94.75
7	28.00	28.00	36.00	92.97 ± 5.06	93.37
8	28.00	20.00	28.00	115.92 ± 2.90	115.53
9	22.00	20.00	32.00	86.35 ± 2.72	85.93
10	28.00	24.00	32.00	124.22 ± 1.24	124.15
11	28.00	24.00	32.00	123.85 ± 1.63	124.15
12	34.00	20.00	32.00	92.67 ± 3.56	93.37
13	34.00	24.00	36.00	90.80 ± 2.90	90.82
14	22.00	24.00	28.00	91.98 ± 3.72	91.95
15	28.00	20.00	36.00	77.48 ± 2.35	77.28

Each experiment was repeated three times, and all of the data were expressed as means ± standard deviations. X₁, X₂, and X₃ represent corn powder (g/L), soybean meal, and incubation temperature (°C), respectively.